TY - JOUR
T1 - Potentials and capabilities of the Extracellular Vesicle (EV) Array
AU - Jørgensen, Malene Møller
AU - Bæk, Rikke
AU - Varming, Kim
PY - 2015
Y1 - 2015
N2 - Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.
AB - Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high-throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma-derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.
U2 - 10.3402/jev.v4.26048
DO - 10.3402/jev.v4.26048
M3 - Journal article
C2 - 25862471
SN - 2001-3078
VL - 4
JO - Journal of Extracellular Vesicles
JF - Journal of Extracellular Vesicles
M1 - 26048
ER -