Prognostic Impact of a 12-gene Progression Score in Non-muscle-invasive Bladder Cancer: A Prospective Multicentre Validation Study

Lars Dyrskjøt, Thomas Reinert, Ferran Algaba, Emil Christensen, Daan Nieboer, Gregers G Hermann, Karin Mogensen, Willemien Beukers, Mirari Marquez, Ulrika Segersten, Søren Høyer, Benedicte Parm Ulhøi, Arndt Hartmann, Robert Stöhr, Sven Wach, Roman Nawroth, Kristina Schwamborn, Cane Tulic, Tatjana Simic, Kerstin JunkerNiels Harving, Astrid C Petersen, Jørgen Bjerggaard Jensen, Bastian Keck, Marc-Oliver Grimm, Marcus Horstmann, Tobias Maurer, Ewout W Steyerberg, Ellen C Zwarthoff, Francisco X Real, Nuria Malats, Per-Uno Malmström, Torben Falck Ørntoft

Research output: Contribution to journalJournal articleResearchpeer-review

68 Citations (Scopus)

Abstract

BACKGROUND: Progression of non-muscle-invasive bladder cancer (NMIBC) to muscle-invasive bladder cancer (MIBC) is life-threatening and cannot be accurately predicted using clinical and pathological risk factors. Biomarkers for stratifying patients to treatment and surveillance are greatly needed.

OBJECTIVE: To validate a previously developed 12-gene progression score to predict progression to MIBC in a large, multicentre, prospective study.

DESIGN, SETTING, AND PARTICIPANTS: We enrolled 1224 patients in ten European centres between 2008 and 2012. A total of 750 patients (851 tumours) fulfilled the inclusion and sample quality criteria for testing. Patients were followed for an average of 28 mo (range 0-76). A 12-gene real-time qualitative polymerase chain reaction assay was performed for all tumours and progression scores were calculated using a predefined formula and cut-off values.

OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: We measured progression to MIBC using Cox regression analysis and log-rank tests for comparing survival distributions.

RESULTS AND LIMITATIONS: The progression score was significantly (p<0.001) associated with age, stage, grade, carcinoma in situ, bacillus Calmette-Guérin treatment, European Organisation for Research and Treatment of Cancer risk score, and disease progression. Univariate Cox regression analysis showed that patients molecularly classified as high risk experienced more frequent disease progression (hazard ratio 5.08, 95% confidence interval 2.2-11.6; p<0.001). Multivariable Cox regression models showed that the progression score added independent prognostic information beyond clinical and histopathological risk factors (p<0.001), with an increase in concordance statistic from 0.82 to 0.86. The progression score showed high correlation (R(2)=0.85) between paired fresh-frozen and formalin-fixed paraffin-embedded tumour specimens, supporting translation potential in the standard clinical setting. A limitation was the relatively low progression rate (5%, 37/750 patients).

CONCLUSIONS: The 12-gene progression score had independent prognostic power beyond clinical and histopathological risk factors, and may help in stratifying NMIBC patients to optimise treatment and follow-up regimens.

PATIENT SUMMARY: Clinical use of a 12-gene molecular test for disease aggressiveness may help in stratifying patients with non-muscle-invasive bladder cancer to optimal treatment regimens.

Original languageEnglish
JournalEuropean Urology
Volume72
Issue number3
Pages (from-to)461-469
Number of pages9
ISSN0302-2838
DOIs
Publication statusPublished - 2017

Keywords

  • Journal Article

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