Proteomic analysis of cytoskeleton proteins in fish

Michael Gotesman; Simon Menanteau-Ledouble

doi:10.1007/978-1-4939-3124-8_21

Proteomic analysis of cytoskeleton proteins in fish

Michael Gotesman
, Simon Menanteau-Ledouble

Research output: Contribution to book/anthology/report/conference proceeding › Book chapter › Research › peer-review

4 Citations (Scopus)

Abstract

In this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. We have used the common carp (Cyprinus carpio L.) and goldfish (Cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. The chapter describes detailed protocols for maintenance of carp, cell culture, antibody purification of proteins, and use of electrospray-ionization mass spectrometry analysis to screen and identify cytoskeleton and other proteins that may be involved in viral infection and propagation in fish.

Original language	English
Title of host publication	Methods in Molecular Biology
Number of pages	16
Publisher	Humana Press, Inc.
Publication date	2016
Pages	357-372
DOIs	https://doi.org/10.1007/978-1-4939-3124-8_21
Publication status	Published - 2016

Series	Methods in Molecular Biology
Volume	1365
ISSN	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.

Keywords

Carp
Cyprinid herpesvirus 3 (CyHV-3)
Cyprinidae fish
Electrospray-ionization mass spectrometry (ESI-MS) analysis
Spring viremia of carp virus (SVCV)

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title = "Proteomic analysis of cytoskeleton proteins in fish",

abstract = "In this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. We have used the common carp (Cyprinus carpio L.) and goldfish (Cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. The chapter describes detailed protocols for maintenance of carp, cell culture, antibody purification of proteins, and use of electrospray-ionization mass spectrometry analysis to screen and identify cytoskeleton and other proteins that may be involved in viral infection and propagation in fish.",

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author = "Michael Gotesman and Simon Menanteau-Ledouble",

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doi = "10.1007/978-1-4939-3124-8\_21",

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AU - Menanteau-Ledouble, Simon

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PY - 2016

Y1 - 2016

N2 - In this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. We have used the common carp (Cyprinus carpio L.) and goldfish (Cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. The chapter describes detailed protocols for maintenance of carp, cell culture, antibody purification of proteins, and use of electrospray-ionization mass spectrometry analysis to screen and identify cytoskeleton and other proteins that may be involved in viral infection and propagation in fish.

AB - In this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. We have used the common carp (Cyprinus carpio L.) and goldfish (Cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. The chapter describes detailed protocols for maintenance of carp, cell culture, antibody purification of proteins, and use of electrospray-ionization mass spectrometry analysis to screen and identify cytoskeleton and other proteins that may be involved in viral infection and propagation in fish.

KW - Carp

KW - Cyprinid herpesvirus 3 (CyHV-3)

KW - Cyprinidae fish

KW - Electrospray-ionization mass spectrometry (ESI-MS) analysis

KW - Spring viremia of carp virus (SVCV)

UR - https://www.scopus.com/pages/publications/84946048638

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M3 - Book chapter

C2 - 26498797

AN - SCOPUS:84946048638

T3 - Methods in Molecular Biology

SP - 357

EP - 372

BT - Methods in Molecular Biology

PB - Humana Press, Inc.

ER -

Proteomic analysis of cytoskeleton proteins in fish

Abstract

Bibliographical note

Keywords

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