TY - JOUR
T1 - Proteomic Changes in Methicillin-Resistant Staphylococcus aureus Exposed to Cannabinoids
AU - Poulsen, Jan Struckmann
AU - Kjærager Nielsen, Christina
AU - Pedersen, Nina Ahrendt
AU - Wimmer, Reinhard
AU - Sondergaard, Teis Esben
AU - Jonge, Nadieh de
AU - Nielsen, Jeppe Lund
PY - 2023/7/28
Y1 - 2023/7/28
N2 - Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen that causes a wide range of infections. Its resistance to β-lactam antibiotics complicates treatment due to the limited number of antibiotics with activity against MRSA. To investigate development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. In this study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds and analyzed using proteomics to assess the changes in physiology. Subjecting MRSA to nonlethal levels of methicillin resulted in an increased production of penicillin-binding protein 2 (PBP2). Exposure to cannabinoids showed antibiotic activity against MRSA, and differential proteomics revealed reduced levels of proteins involved in the energy production as well as PBP2 when used in combination with methicillin.
AB - Methicillin-resistant Staphylococcus aureus (MRSA) is a major human pathogen that causes a wide range of infections. Its resistance to β-lactam antibiotics complicates treatment due to the limited number of antibiotics with activity against MRSA. To investigate development of alternative therapeutics, the mechanisms that mediate antibiotic resistance in MRSA need to be fully understood. In this study, MRSA cells were subjected to antibiotic stress from methicillin in combination with three cannabinoid compounds and analyzed using proteomics to assess the changes in physiology. Subjecting MRSA to nonlethal levels of methicillin resulted in an increased production of penicillin-binding protein 2 (PBP2). Exposure to cannabinoids showed antibiotic activity against MRSA, and differential proteomics revealed reduced levels of proteins involved in the energy production as well as PBP2 when used in combination with methicillin.
UR - http://www.scopus.com/inward/record.url?scp=85164710029&partnerID=8YFLogxK
U2 - 10.1021/acs.jnatprod.3c00064
DO - 10.1021/acs.jnatprod.3c00064
M3 - Journal article
SN - 0163-3864
VL - 86
SP - 1690
EP - 1697
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 7
ER -