Projects per year
Here we demonstrate how quantitative proteomics by LC-MS/MS is a highly valuable tool for characterization and evaluation of protein extracts (Figure 1). In contrast to conventional protein characterization in food science, proteomics provides a deeper level of insight on the quantitative composition. By applying down-stream bioinformatic analysis, it is possible to predict subcellular localization of individual proteins. Such information was used to quantitatively evaluate efficiency and selectivity in different extraction methods, which is possible even in quite poorly characterized species like Spinosum. We evaluated several Spinosum extracts and found that pre-treatment with cell wall degrading enzymes resulted in higher protein content due to increased intracellular protein recovery compared to other extraction methods - without negative impact on yield nor quality of extracted carrageenan. Furthermore, using novel bioinformatic algorithms we predicted a multitude of peptides with emulsifying and antioxidant properties, embedded in abundantly extracted proteins. These functionalities were validated in vitro for many predicted peptides, increasing potential applicability and value. Finally, proteomics data were evaluated as a future replacement of conventional amino acid analysis for evaluating nutritional value.
|Publication date||27 Sep 2021|
|Number of pages||1|
|Publication status||Published - 27 Sep 2021|
|Event||10th Nordic Seaweed Conference: The next 10 years - from local to global - Kysthotellet, Grenaa, Denmark|
Duration: 13 Oct 2021 → 14 Oct 2021
|Conference||10th Nordic Seaweed Conference|
|Period||13/10/2021 → 14/10/2021|
FingerprintDive into the research topics of 'Quantitative proteomics and bioinformatics in seaweed food protein research: Evaluation of extraction methods, bioactive potential, and nutritional value.'. Together they form a unique fingerprint.
- 1 Active
01/09/2017 → 30/09/2022
- 1 Conference presentations
Quantitative proteomics and bioinformatics in seaweed food protein research: Evaluation of extraction methods, bioactive potential, and nutritional value.
Simon Gregersen (Lecturer)14 Oct 2021
Activity: Talks and presentations › Conference presentations
- 1 Conference abstract for conference
Applying Quantitative Proteomics for Evaluation of Protein Quality, Nutritional Value, and Extraction Methods in Side-Streams of Industrial Carrageenan Production from the Red Seaweed Eucheuma denticulatum (Spinosum)Gregersen, S., Yesiltas, B., García Moreno, P. J., Naseri, A., Holdt, S. L., Jacobsen, C., Hansen, E. B., Marcatili, P. & Overgaard, M. T., 1 Oct 2021.
Research output: Contribution to conference without publisher/journal › Conference abstract for conference › Research › peer-review