Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)

Reza Ghanei-Motlagh; Takavar Mohammadian; Darioush Gharibi; Mohammad Khosravi; Esmaeil Mahmoudi; Mojtaba Zarea; Mansour El-Matbouli; Simon Menanteau-Ledouble

doi:10.1016/j.aquaculture.2020.735874

Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)

Reza Ghanei-Motlagh^*, Takavar Mohammadian, Darioush Gharibi, Mohammad Khosravi, Esmaeil Mahmoudi, Mojtaba Zarea, Mansour El-Matbouli, Simon Menanteau-Ledouble

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

49 Citations (Scopus)

Abstract

The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 ± 0.6 g and 15.5 ± 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 × 10⁹ CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (α-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.

Original language	English
Article number	735874
Journal	Aquaculture
Volume	531
ISSN	0044-8486
DOIs	https://doi.org/10.1016/j.aquaculture.2020.735874
Publication status	Published - 30 Jan 2021
Externally published	Yes

Bibliographical note

Funding Information:
This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299 ). The funding body had no role in the design of the study or interpretation of data.

Funding Information:
This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299). The funding body had no role in the design of the study or interpretation of data.

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

Asian seabass
Bacillus
Performance
Quorum quenching probiotic
Survival
Vibrio harveyi

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Ghanei-Motlagh, R., Mohammadian, T., Gharibi, D., Khosravi, M., Mahmoudi, E., Zarea, M., El-Matbouli, M., & Menanteau-Ledouble, S. (2021). Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer). Aquaculture, 531, Article 735874. https://doi.org/10.1016/j.aquaculture.2020.735874

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title = "Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)",

abstract = "The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 ± 0.6 g and 15.5 ± 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 × 109 CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (α-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.",

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author = "Reza Ghanei-Motlagh and Takavar Mohammadian and Darioush Gharibi and Mohammad Khosravi and Esmaeil Mahmoudi and Mojtaba Zarea and Mansour El-Matbouli and Simon Menanteau-Ledouble",

note = "Funding Information: This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299 ). The funding body had no role in the design of the study or interpretation of data. Funding Information: This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299). The funding body had no role in the design of the study or interpretation of data. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

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month = jan,

day = "30",

doi = "10.1016/j.aquaculture.2020.735874",

language = "English",

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Ghanei-Motlagh, R, Mohammadian, T, Gharibi, D, Khosravi, M, Mahmoudi, E, Zarea, M, El-Matbouli, M & Menanteau-Ledouble, S 2021, 'Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)', Aquaculture, vol. 531, 735874. https://doi.org/10.1016/j.aquaculture.2020.735874

Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer). / Ghanei-Motlagh, Reza; Mohammadian, Takavar; Gharibi, Darioush et al.
In: Aquaculture, Vol. 531, 735874, 30.01.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)

AU - Ghanei-Motlagh, Reza

AU - Mohammadian, Takavar

AU - Gharibi, Darioush

AU - Khosravi, Mohammad

AU - Mahmoudi, Esmaeil

AU - Zarea, Mojtaba

AU - El-Matbouli, Mansour

AU - Menanteau-Ledouble, Simon

N1 - Funding Information: This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299 ). The funding body had no role in the design of the study or interpretation of data. Funding Information: This research was financed by a grant from Shahid Chamran University of Ahvaz Research Council (Grant No. vC98.299). The funding body had no role in the design of the study or interpretation of data. Publisher Copyright: © 2020 Elsevier B.V.

PY - 2021/1/30

Y1 - 2021/1/30

N2 - The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 ± 0.6 g and 15.5 ± 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 × 109 CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (α-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.

AB - The use of probiotics is a promising and increasingly common strategy to maintain a healthy farming environment in an eco-friendly and sustainable manner. The goal of this study was to investigate the possible effects of dietary supplementation with autochthonous quorum quenching (QQ) probiotics on growth, digestive enzymes activity, haemato-biochemical responses, gut microbial communities and resistance against Vibrio harveyi in Asian seabass. Asian seabass (75 ± 0.6 g and 15.5 ± 0.3 cm) were fed with basal diet (control groups) and basal diet containing 1 × 109 CFU per g Bacillus thuringiensis QQ1 or Bacillus cereus QQ2 for 6 weeks. Feeding with diets supplemented with QQ probiotics for 35 days (pre-infection period) resulted in a significant (p < 0.05) alteration of the growth parameters (RGR, FCR, SGR, PER, FER and FI) and digestive enzymes activity (α-amylase, total protease, lipase, trypsin, alkaline phosphatase, T/C and A/T). Hematocrit (Hct) and total leucocyte count (TLC) were significantly (p < 0.05) elevated in fish fed with QQ1. Respiratory burst activity (RBA) likewise significantly (p < 0.05) increased in the probiotic groups relative to the control groups. Moreover, a significant decrease (p < 0.05) in the level of serum total cholesterol was observed in fish fed with probiotic. QQ1 and QQ2 were able to colonize the host's intestine at high number after 5 weeks. Hct and RBA in QQ1 and QQ2, globulin in QQ2 and TLC in QQ1 were significantly (p < 0.05) promoted compared to the positive control (PC) after infection with Vibrio harveyi (day 42). Serum triglycerides, cholesterol, alkaline phosphatase (ALP), alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and lactate dehydrogenase (LDH) were significantly (p < 0.05) lower in the probiotic treatments than the PC group after infection. QQ probionts significantly (p < 0.05) improved the cumulative survival of Asian seabass against V. harveyi. According to these findings, we suggest that B. cereus QQ2 and particularly B. thuringiensis QQ1 have a high potential to enhance the performances of Asian seabass and therefore can be effectively applied for the control of V. harveyi.

KW - Asian seabass

KW - Bacillus

KW - Performance

KW - Quorum quenching probiotic

KW - Survival

KW - Vibrio harveyi

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DO - 10.1016/j.aquaculture.2020.735874

M3 - Journal article

AN - SCOPUS:85089909458

SN - 0044-8486

VL - 531

JO - Aquaculture

JF - Aquaculture

M1 - 735874

ER -

Quorum quenching probiotics modulated digestive enzymes activity, growth performance, gut microflora, haemato-biochemical parameters and resistance against Vibrio harveyi in Asian seabass (Lates calcarifer)

Abstract

Bibliographical note

Keywords

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