Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells

Fang Wang; Yasuko Maeda; Vladimir Zachar; Tahera Ansari; Jeppe Emmersen

doi:10.1016/j.bbrc.2018.06.014

Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells

Fang Wang, Yasuko Maeda, Vladimir Zachar, Tahera Ansari, Jeppe Emmersen

Research output: Contribution to journal › Journal article › Research › peer-review

16 Citations (Scopus)

Abstract

This study explored the feasibility of constructing a tissue engineered muscle layer in the oesophagus using oesophageal acellular matrix (OAM) scaffolds and human aortic smooth muscle cells (hASMCs) or human adipose-derived stem cells (hASCs). The second objective was to investigate the effect of hypoxic preconditioning of seeding cells on cell viability and migration depth. Our results demonstrated that hASMCs and hASCs could attach and adhere to the decellularized OAM scaffold and survive and proliferate for at least 7 days depending on the growth conditions. This indicates adipose-derived stem cells (ASCs) have the potential to substitute for smooth muscle cells (SMCs) in the construction of tissue engineered oesophageal muscle layers.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	503
Issue number	1
Pages (from-to)	271-277
Number of pages	7
ISSN	0006-291X
DOIs	https://doi.org/10.1016/j.bbrc.2018.06.014
Publication status	Published - 3 Sept 2018

Keywords

Adipose-derived stem cells
Differentiation
Hypoxia
Mesenchymal stem cells
Oesophageal acellular matrix scaffold
Oesophageal tissue engineering
Smooth muscle cells

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title = "Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells",

abstract = "This study explored the feasibility of constructing a tissue engineered muscle layer in the oesophagus using oesophageal acellular matrix (OAM) scaffolds and human aortic smooth muscle cells (hASMCs) or human adipose-derived stem cells (hASCs). The second objective was to investigate the effect of hypoxic preconditioning of seeding cells on cell viability and migration depth. Our results demonstrated that hASMCs and hASCs could attach and adhere to the decellularized OAM scaffold and survive and proliferate for at least 7 days depending on the growth conditions. This indicates adipose-derived stem cells (ASCs) have the potential to substitute for smooth muscle cells (SMCs) in the construction of tissue engineered oesophageal muscle layers.",

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AU - Maeda, Yasuko

AU - Zachar, Vladimir

AU - Ansari, Tahera

AU - Emmersen, Jeppe

PY - 2018/9/3

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KW - Mesenchymal stem cells

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Regeneration of the oesophageal muscle layer from oesophagus acellular matrix scaffold using adipose-derived stem cells

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