RN7SL1 may be translated under oncogenic conditions

Tomoaki Hara; Sikun Meng; Yoshiko Tsuji; Yasuko Arao; Yoshiko Saito; Hiromichi Sato; Daisuke Motooka; Shizuka Uchida; Hideshi Ishii

doi:10.1073/pnas.2312322121

RN7SL1 may be translated under oncogenic conditions

Tomoaki Hara^*, Sikun Meng, Yoshiko Tsuji, Yasuko Arao, Yoshiko Saito, Hiromichi Sato, Daisuke Motooka, Shizuka Uchida, Hideshi Ishii^*

^*Corresponding author for this work

Research output: Contribution to journal › Letter › peer-review

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Abstract

RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.

Original language	English
Article number	e2312322121
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	121
Issue number	12
Pages (from-to)	e2312322121
Number of pages	3
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.2312322121
Publication status	Published - 19 Mar 2024

Keywords

Green Fluorescent Proteins/genetics
Peptides/metabolism
Signal Recognition Particle/metabolism
ncRNA
smORF
RN7SL1

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Hara et al. (2024). RN7SL1 may be translated under oncogenic conditionsFinal published version, 389 KBLicence: CC BY-NC-ND 4.0

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abstract = "RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.",

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AU - Hara, Tomoaki

AU - Meng, Sikun

AU - Tsuji, Yoshiko

AU - Arao, Yasuko

AU - Saito, Yoshiko

AU - Sato, Hiromichi

AU - Motooka, Daisuke

AU - Uchida, Shizuka

AU - Ishii, Hideshi

PY - 2024/3/19

Y1 - 2024/3/19

N2 - RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.

AB - RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.

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KW - Peptides/metabolism

KW - Signal Recognition Particle/metabolism

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RN7SL1 may be translated under oncogenic conditions

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Keywords

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