Abstract
Here we describe a new method applying phage-displayed antibody libraries to the selection of antibodies against a single identified cell on a glass slide. this is the only described method that has successfully achieved selection of antibodies against a single rare cell in a heterogeneous population of cells. the phage library is incubated with the slide containing the identified rare cell of interest; incubation is followed by uV irradiation while protecting the target cell with a minute disc. the uV light inactivates all phages outside the shielded area by cross-linking the Dnaconstituting their genomes. the expected yield is between one and ten phage particles from a single cell selection. the encoded antibodies are subsequently produced monoclonally and tested for specificity. this method can be applied within a week to carry out ten or more individual cell selections. Including subsequent testing of antibody specificity, a specific antibody can be identified within 2 months.
Original language | English |
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Journal | Nature Protocols |
Volume | 6 |
Issue number | 4 |
Pages (from-to) | 509-522 |
Number of pages | 14 |
ISSN | 1754-2189 |
DOIs | |
Publication status | Published - 1 Jan 2011 |
Externally published | Yes |