SELECTION SYSTEM FOR PHAGEMIDS USING PROTEOLYTICALLY SENSITIVE HELPER PHAGE

Riechmann Lutz (Inventor), Kristensen Peter (Inventor), Jestin Jean-Luc (Inventor), Winter Gregory Paul (Inventor), Riechmann Lutz * (Inventor), Kristensen Peter * (Inventor), Jestin Jean-Luc * (Inventor), Winter Gregory Paul * (Inventor)

Research output: Patent

Abstract

A method for effecting helper phage rescue of a phagemid comprising a fusion polypeptide to form progeny bacteriophage wherein

(a) the helper phage encodes a viral coat protein comprising a protease sensitive cleavage site (b) the phagemid comprises a fusion polypeptide (c) the progeny phage are exposed to a protease capable of cleaving the proteolytically cleavable site such that the cleavage of the protein derived from the helper phage impairs its ability to mediate infection and (d) the progeny are propagated by infection. The helper phage has the protease sensitive site within p3 coat protein and is the filamentous bacteriophage M13, fd or a related species.
Original languageEnglish
IPCC40B 40/ 02 A I
Patent numberGB2379933
CountryDenmark
Priority date13/05/1999
Priority numberGB20000026143
Publication statusPublished - 26 Mar 2003

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