Self-assembled nanoparticles based on cyclodextrin-modified pullulan: Synthesis, and structural characterization using SAXS

Jakob Stensgaard Diget, Reidar Lund, Bo Nyström, Véronique Wintgens, Catherine Amiel, Reinhard Wimmer, Thorbjørn Terndrup Nielsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

15 Citations (Scopus)
225 Downloads (Pure)

Abstract

Synthesis of novel host-guest functionalized polymers is presented along with structural characterization using small-angle X-ray scattering (SAXS) of the resulting nanoparticles. Mono-6-deoxy-mono-6-azidoβCD (N 3 βCD) was grafted onto alkyne-functionalized pullulan via the “click” reaction copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) and an adamantane-modified dextran was prepared via the same strategy. Characterization of the polymers was carried out using nuclear magnetic resonance (NMR) spectroscopy, gel filtration chromatography (GFC), isothermal titration calorimetry (ITC) and SAXS. Nanoparticles were created via host-guest interactions between the well-defined βCD-pullulans and adamantane-modified dextran. Characterization was carried out using dynamic light scattering (DLS) and SAXS, which revealed spherical particles in the sub-100 nm range. The studies shed light on the importance of molecular structure and host-guest ratio on crucial properties such as particle size, size distribution, porosity and stability towards aggregation.

Original languageEnglish
JournalCarbohydrate Polymers
Volume213
Pages (from-to)403-410
Number of pages8
ISSN0144-8617
DOIs
Publication statusPublished - 1 Jun 2019

Keywords

  • Host-guest chemistry
  • Pullulan
  • Self-assembly
  • Small-angle X-ray scattering (SAXS)
  • β-Cyclodextrin (βCD)
  • “Click” chemistry

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