Serum Lipoprotein Profiling by NMR Spectroscopy Reveals Alterations in HDL-1 and HDL-2 Apo-A2 Subfractions in Alzheimer's Disease

Jonas Ellegaard Mortensen, Trygve Andreassen, Dorte Aalund Olsen, Karsten Vestergaard, Jonna Skov Madsen, Søren Risom Kristensen, Shona Pedersen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Identifying biomarkers for Alzheimer's disease (AD) is crucial, due to its complex pathology, which involves dysfunction in lipid transport, contributing to neuroinflammation, synaptic loss, and impaired amyloid-β clearance. Nuclear magnetic resonance (NMR) is able to quantify and stratify lipoproteins. The study investigated lipoproteins in blood from AD patients, aiming to evaluate their diagnostic potential. Serum and plasma were collected from AD patients (n = 25) and healthy individuals (n = 25). We conducted a comprehensive lipoprotein profiling on serum samples using NMR spectroscopy, analysing 112 lipoprotein subfractions. In plasma, we measured unspecific markers of neuronal damage and AD hallmark proteins using single molecule array technology. Additionally, clinical data and cerebrospinal fluid biomarker levels were also collected to enrich our data. Our findings, after adjusting for age and sex differences, highlight significant alterations in two specific lipoproteins; high-density lipoprotein (HDL)-1 Apo-A2 (H1A2) and HDL-2 Apo-A2 (H2A2), both with area under the curve (AUC) values of 0.67, 95% confidence interval (CI) = 0.52-0.82). These results indicate that these lipoprotein subfractions may have potential as indicators of AD-related metabolic changes.

Original languageEnglish
Article number11701
JournalInternational Journal of Molecular Sciences
Volume25
Issue number21
Number of pages13
ISSN1661-6596
DOIs
Publication statusPublished - 1 Nov 2024

Keywords

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease/blood
  • Apolipoprotein A-II/blood
  • Biomarkers/blood
  • Female
  • Humans
  • Lipoproteins, HDL/blood
  • Lipoproteins, HDL2/blood
  • Magnetic Resonance Spectroscopy/methods
  • Male
  • Middle Aged
  • Alzheimer’s disease
  • biomarker
  • blood
  • nuclear magnetic resonance
  • serum
  • lipoproteins

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