TY - JOUR
T1 - Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin
AU - Dyring-Andersen, Beatrice
AU - Løvendorf, Marianne Bengtson
AU - Coscia, Fabian
AU - Santos, Alberto
AU - Møller, Line Bruun Pilgaard
AU - Colaço, Ana R
AU - Niu, Lili
AU - Bzorek, Michael
AU - Doll, Sophia
AU - Andersen, Jørgen Lock
AU - Clark, Rachael A
AU - Skov, Lone
AU - Teunissen, Marcel B M
AU - Mann, Matthias
PY - 2020/11/5
Y1 - 2020/11/5
N2 - Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantify 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe the quantitative distribution of structural proteins, known and previously undescribed proteins specific to cellular subsets and those with specialized immunological functions such as cytokines and chemokines. We anticipate that this proteomic atlas of human skin will become an essential community resource for basic and translational research ( https://skin.science/ ).
AB - Human skin provides both physical integrity and immunological protection from the external environment using functionally distinct layers, cell types and extracellular matrix. Despite its central role in human health and disease, the constituent proteins of skin have not been systematically characterized. Here, we combine advanced tissue dissection methods, flow cytometry and state-of-the-art proteomics to describe a spatially-resolved quantitative proteomic atlas of human skin. We quantify 10,701 proteins as a function of their spatial location and cellular origin. The resulting protein atlas and our initial data analyses demonstrate the value of proteomics for understanding cell-type diversity within the skin. We describe the quantitative distribution of structural proteins, known and previously undescribed proteins specific to cellular subsets and those with specialized immunological functions such as cytokines and chemokines. We anticipate that this proteomic atlas of human skin will become an essential community resource for basic and translational research ( https://skin.science/ ).
UR - http://www.scopus.com/inward/record.url?scp=85095411628&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-19383-8
DO - 10.1038/s41467-020-19383-8
M3 - Journal article
C2 - 33154365
SN - 2041-1723
VL - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 5587
ER -