TY - JOUR
T1 - Statistical optimization of biohydrogen and ethanol production from crude glycerol by microbial mixed culture
AU - Varrone, Cristiano
AU - Giussani, Barbara
AU - Izzo, Giulio
AU - Massini, Giulia
AU - Marone, Antonella
AU - Signorini, Antonella
AU - Wang, Aijie
PY - 2012/11
Y1 - 2012/11
N2 - Design of Experiments (DoE) was applied to improve the ability of enriched activity sludge to efficiently convert crude glycerol from biodiesel industry into hydrogen and ethanol, using a very simple synthetic medium. Based on Plackett-Burman screening design, glycerol concentration, temperature and initial pH were identified as significant variables. Box-Behnken design and Response Surface Method (RSM) were then used for optimization. The maximum hydrogen yield of 0.96 mol H 2/mol glycerol was estimated at the temperature of 37.0 °C, initial pH of 7.9 and glycerol concentration of 15.0 g/L. Maximum hydrogen production rate of 2191 mL/L/d was estimated at the temperature of 37.3 °C, initial pH of 8.0 and glycerol concentration of 15.2 g/L. Finally maximum ethanol production of 7.92 g/L was estimated at an initial pH of 8.0 and glycerol concentration of 15.0 g/L (temperature had no significant effect). These results show that it is possible to obtain both, high yield and production of hydrogen and ethanol together, using a very simple synthetic medium, without trace element- and vitamin solution, tryptone or yeast extract.
AB - Design of Experiments (DoE) was applied to improve the ability of enriched activity sludge to efficiently convert crude glycerol from biodiesel industry into hydrogen and ethanol, using a very simple synthetic medium. Based on Plackett-Burman screening design, glycerol concentration, temperature and initial pH were identified as significant variables. Box-Behnken design and Response Surface Method (RSM) were then used for optimization. The maximum hydrogen yield of 0.96 mol H 2/mol glycerol was estimated at the temperature of 37.0 °C, initial pH of 7.9 and glycerol concentration of 15.0 g/L. Maximum hydrogen production rate of 2191 mL/L/d was estimated at the temperature of 37.3 °C, initial pH of 8.0 and glycerol concentration of 15.2 g/L. Finally maximum ethanol production of 7.92 g/L was estimated at an initial pH of 8.0 and glycerol concentration of 15.0 g/L (temperature had no significant effect). These results show that it is possible to obtain both, high yield and production of hydrogen and ethanol together, using a very simple synthetic medium, without trace element- and vitamin solution, tryptone or yeast extract.
KW - Biodiesel
KW - Bioethanol
KW - Biohydrogen
KW - Crude glycerol
KW - Dark fermentation
KW - Statistical optimization
UR - http://www.scopus.com/inward/record.url?scp=84867398432&partnerID=8YFLogxK
U2 - 10.1016/j.ijhydene.2012.02.106
DO - 10.1016/j.ijhydene.2012.02.106
M3 - Journal article
AN - SCOPUS:84867398432
SN - 0360-3199
VL - 37
SP - 16479
EP - 16488
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 21
ER -