Abstract
Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many
crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene
function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be
used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces
cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani
f. sp. pisi.
crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene
function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be
used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces
cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani
f. sp. pisi.
| Original language | English |
|---|---|
| Title of host publication | Engineering Natural Product Biosynthesis : Methods and Protocols |
| Editors | Elizabeth Skellam |
| Number of pages | 22 |
| Publisher | Springer |
| Publication date | 7 May 2022 |
| Pages | 93-114 |
| Chapter | 6 |
| ISBN (Print) | 978-1-0716-2272-8 |
| ISBN (Electronic) | 978-1-0716-2273-5 |
| DOIs | |
| Publication status | Published - 7 May 2022 |
| Series | Methods in Molecular Biology |
|---|---|
| Volume | 2489 |
| ISSN | 1064-3745 |
Keywords
- AMT
- ATMT
- Agrobacterium-mediated transformation
- Filamentous fungi
- Fusarium
- Fusarium solani
- Gene targeting
- Genomic integration
- Knockout
- Overexpression