Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani

Mikkel Rank Nielsen; Samba Eveline Kabemba Kaniki; Jens Laurids Sørensen

doi:10.1007/978-1-0716-2273-5_6

Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani

Mikkel Rank Nielsen^*
, Samba Eveline Kabemba Kaniki
, Jens Laurids Sørensen

^*Corresponding author for this work

Research output: Contribution to book/anthology/report/conference proceeding › Book chapter › Research › peer-review

2 Citations (Scopus)

Abstract

Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many
crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene
function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be
used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces
cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani
f. sp. pisi.

Original language	English
Title of host publication	Engineering Natural Product Biosynthesis : Methods and Protocols
Editors	Elizabeth Skellam
Number of pages	22
Publisher	Springer
Publication date	7 May 2022
Pages	93-114
Chapter	6
ISBN (Print)	978-1-0716-2272-8
ISBN (Electronic)	978-1-0716-2273-5
DOIs	https://doi.org/10.1007/978-1-0716-2273-5_6
Publication status	Published - 7 May 2022

Series	Methods in Molecular Biology
Volume	2489
ISSN	1064-3745

Keywords

AMT
ATMT
Agrobacterium-mediated transformation
Filamentous fungi
Fusarium
Fusarium solani
Gene targeting
Genomic integration
Knockout
Overexpression

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10.1007/978-1-0716-2273-5_6

AUB Link

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Cite this

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title = "Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani",

abstract = "Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani f. sp. pisi.",

keywords = "AMT, ATMT, Agrobacterium-mediated transformation, Filamentous fungi, Fusarium, Fusarium solani, Gene targeting, Genomic integration, Knockout, Overexpression",

author = "Nielsen, \{Mikkel Rank\} and Kaniki, \{Samba Eveline Kabemba\} and S{\o}rensen, \{Jens Laurids\}",

year = "2022",

month = may,

day = "7",

doi = "10.1007/978-1-0716-2273-5\_6",

language = "English",

isbn = "978-1-0716-2272-8",

series = "Methods in Molecular Biology",

publisher = "Springer",

pages = "93--114",

editor = "Elizabeth Skellam",

booktitle = "Engineering Natural Product Biosynthesis",

address = "Germany",

}

Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani. / Nielsen, Mikkel Rank; Kaniki, Samba Eveline Kabemba; Sørensen, Jens Laurids.
Engineering Natural Product Biosynthesis: Methods and Protocols. ed. / Elizabeth Skellam. Springer, 2022. p. 93-114 (Methods in Molecular Biology, Vol. 2489).

Research output: Contribution to book/anthology/report/conference proceeding › Book chapter › Research › peer-review

TY - CHAP

T1 - Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani

AU - Nielsen, Mikkel Rank

AU - Kaniki, Samba Eveline Kabemba

AU - Sørensen, Jens Laurids

PY - 2022/5/7

Y1 - 2022/5/7

N2 - Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani f. sp. pisi.

AB - Members of the Fusarium solani species complex are filamentous fungi that can act as pathogens to many crops and animals. Although relevant, a robust molecular toolbox is missing for the investigation of gene function and metabolism. In this chapter, we describe how Agrobacterium-mediated transformation can be used to facilitate gene targeting. A flexible vector system, based on in vivo recombination in Saccharomyces cerevisiae, is utilized to achieve overexpression and gene deletion of targeted biosynthetic genes in F. solani f. sp. pisi.

KW - AMT

KW - ATMT

KW - Agrobacterium-mediated transformation

KW - Filamentous fungi

KW - Fusarium

KW - Fusarium solani

KW - Gene targeting

KW - Genomic integration

KW - Knockout

KW - Overexpression

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SP - 93

EP - 114

BT - Engineering Natural Product Biosynthesis

A2 - Skellam, Elizabeth

PB - Springer

ER -

Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani

Abstract

Keywords

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FungalBat: Fungal batteries for storing sustainable energy

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Targeted Genetic Engineering via Agrobacterium-Mediated Transformation in Fusarium solani

Abstract

Keywords

Access to Document

AUB Link

Other files and links

Projects

FungalBat: Fungal batteries for storing sustainable energy

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