The influence of glucosylation on the renaturation rate of T4 phage DNA

C. Christiansen; G. Christiansen; A. Leth Bak

doi:10.1016/0006-291X(73)90660-8

The influence of glucosylation on the renaturation rate of T4 phage DNA

C. Christiansen^*, G. Christiansen, A. Leth Bak

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

7 Citations (Scopus)

Abstract

The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	52
Issue number	4
Pages (from-to)	1426-1433
Number of pages	8
ISSN	0006-291X
DOIs	https://doi.org/10.1016/0006-291X(73)90660-8
Publication status	Published - 19 Jun 1973
Externally published	Yes

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title = "The influence of glucosylation on the renaturation rate of T4 phage DNA",

abstract = "The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.",

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AU - Christiansen, C.

AU - Christiansen, G.

AU - Bak, A. Leth

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Y1 - 1973/6/19

N2 - The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.

AB - The kinetic complexity of T4 phage DNA with different degrees of glucosylation was determined by studies of DNA renaturation. It was found that this parameter decreased with decreasing degree of glucosylation, and that the kinetic complexity of non-glucosylated DNA was in agreement with the known genome size of T4 phage. This observation indicates that no significant correction for differences in GC content is necessary in the determination of genome sizes from renaturation data.

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U2 - 10.1016/0006-291X(73)90660-8

DO - 10.1016/0006-291X(73)90660-8

M3 - Journal article

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AN - SCOPUS:0015829978

SN - 0006-291X

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EP - 1433

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

The influence of glucosylation on the renaturation rate of T4 phage DNA

Abstract

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