TY - JOUR
T1 - There it is! Fusarium pseudograminearum did not lose the fusaristatin gene cluster after all
AU - Wollenberg, Rasmus Dam
AU - Sondergaard, Teis Esben
AU - Nielsen, Mikkel Rank
AU - Knutsson, Simon
AU - Pedersen, Tobias Bruun
AU - Westphal, Klaus Ringsborg
AU - Wimmer, Reinhard
AU - Gardiner, Donald Max
AU - Sørensen, Jens Laurids
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Fusarium pseudograminearum is a significant pathogen of cereals in arid regions worldwide and has the ability to produce numerous bioactive secondary metabolites. The genome sequences of seven F. pseudograminearum strains have been published and in one of these strains, C5834, we identified an intact gene cluster responsible for biosynthesis of the cyclic lipopeptide fusaristatin A. The high level of sequence identity of the fusaristatin cluster remnant in strains that do not produce fusaristatin suggests that the absence of the cluster evolved once, and subsequently the resulting locus with the cluster fragments became widely dispersed among strains of F. pseudograminearum in Australia. We examined a selection of 99 Australian F. pseudograminearum isolates to determine how widespread the ability to produce fusaristatin A is in F. pseudograminearum. We identified 15 fusaristatin producing strains, all originating from Western Australia. Phylogenetic analyses could not support a division of F. pseudograminearum into fusaristatin producing and nonproducing populations, which could indicate the loss has occurred relatively recent.
AB - Fusarium pseudograminearum is a significant pathogen of cereals in arid regions worldwide and has the ability to produce numerous bioactive secondary metabolites. The genome sequences of seven F. pseudograminearum strains have been published and in one of these strains, C5834, we identified an intact gene cluster responsible for biosynthesis of the cyclic lipopeptide fusaristatin A. The high level of sequence identity of the fusaristatin cluster remnant in strains that do not produce fusaristatin suggests that the absence of the cluster evolved once, and subsequently the resulting locus with the cluster fragments became widely dispersed among strains of F. pseudograminearum in Australia. We examined a selection of 99 Australian F. pseudograminearum isolates to determine how widespread the ability to produce fusaristatin A is in F. pseudograminearum. We identified 15 fusaristatin producing strains, all originating from Western Australia. Phylogenetic analyses could not support a division of F. pseudograminearum into fusaristatin producing and nonproducing populations, which could indicate the loss has occurred relatively recent.
KW - Evolution
KW - Fusarium crown rot
KW - Non-ribosomal peptides
KW - Polyketides
KW - Secondary metabolites
UR - http://www.scopus.com/inward/record.url?scp=85056165316&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2018.10.004
DO - 10.1016/j.funbio.2018.10.004
M3 - Journal article
AN - SCOPUS:85056165316
SN - 1878-6146
VL - 123
SP - 10
EP - 17
JO - Fungal Biology
JF - Fungal Biology
IS - 1
ER -