Transcriptional and Post-Transcriptional Regulation and Transcriptional Memory of Chromatin Regulators in Response to Low Temperature

Kora Vyse, Léa Faivre, Melissa Romich, Majken Pagter, Daniel Schubert, Dirk K. Hincha, Ellen Zuther*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

26 Citations (Scopus)
47 Downloads (Pure)

Abstract

Chromatin regulation ensures stable repression of stress-inducible genes under non-stress conditions and transcriptional activation and memory of stress-related genes after stress exposure. However, there is only limited knowledge on how chromatin genes are regulated at the transcriptional and post-transcriptional level upon stress exposure and relief from stress. We reveal that the repressive modification histone H3 lysine 27 trimethylation (H3K27me3) targets genes which are quickly activated upon cold exposure, however, H3K27me3 is not necessarily lost during a longer time in the cold. In addition, we have set-up a quantitative reverse transcription polymerase chain reaction-based platform for high-throughput transcriptional profiling of a large set of chromatin genes. We find that the expression of many of these genes is regulated by cold. In addition, we reveal an induction of several DNA and histone demethylase genes and certain histone variants after plants have been shifted back to ambient temperature (deacclimation), suggesting a role in the memory of cold acclimation. We also re-analyze large scale transcriptomic datasets for transcriptional regulation and alternative splicing (AS) of chromatin genes, uncovering an unexpected level of regulation of these genes, particularly at the splicing level. This includes several vernalization regulating genes whose AS may result in cold-regulated protein diversity. Overall, we provide a profiling platform for the analysis of chromatin regulatory genes and integrative analyses of their regulation, suggesting a dynamic regulation of key chromatin genes in response to low temperature stress.

Original languageEnglish
Article number39
JournalFrontiers in Plant Science
Volume11
Number of pages18
DOIs
Publication statusPublished - 7 Feb 2020

Bibliographical note

Copyright © 2020 Vyse, Faivre, Romich, Pagter, Schubert, Hincha and Zuther.

Keywords

  • chromatin regulators
  • cold acclimation
  • deacclimation
  • histones
  • quantitative reverse transcription polymerase chain reaction platform

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