Abstract
The Penicillium genus is a valuable collection of ascomycetous fungi that have widespread occurrence in natural environments such as soil. They have attracted much interest due to their ability to produce a wide range of secondary metabolites with importance in the food and pharmaceutical industry, but the full biosynthetic potential of these fungi is yet to be characterized.
A pangenome combines all the genetic information of a set of related organisms and divides this into a set of core genes (genes present in all individuals), soft core genes (genes present in ≥ 95% of all individuals), accessory genes (genes present in < 95% of all individuals, but still at least two individuals), and singleton genes (present in one individual only). Improvements in DNA sequencing technologies have resulted in high quality genome draft assemblies, which can be used to create pangenomes. Since many genes involved in the production of secondary metabolites have been observed to be part of the accessory genome, pangenome characterization is therefore highly useful to explore the biosynthetic potential to produce secondary metabolites.
High molecular DNA was extracted from 94 different Penicillium species and subsequently sequenced in-house using MinION sequencing to create high quality genome drafts. Together with additional ten genome assemblies from NCBI, the collection of species covers diversity of the genus of Penicillium well and forms the basis of a pangenome analysis of the Penicillium genus.
A pangenome combines all the genetic information of a set of related organisms and divides this into a set of core genes (genes present in all individuals), soft core genes (genes present in ≥ 95% of all individuals), accessory genes (genes present in < 95% of all individuals, but still at least two individuals), and singleton genes (present in one individual only). Improvements in DNA sequencing technologies have resulted in high quality genome draft assemblies, which can be used to create pangenomes. Since many genes involved in the production of secondary metabolites have been observed to be part of the accessory genome, pangenome characterization is therefore highly useful to explore the biosynthetic potential to produce secondary metabolites.
High molecular DNA was extracted from 94 different Penicillium species and subsequently sequenced in-house using MinION sequencing to create high quality genome drafts. Together with additional ten genome assemblies from NCBI, the collection of species covers diversity of the genus of Penicillium well and forms the basis of a pangenome analysis of the Penicillium genus.
| Original language | English |
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| Publication date | 15 Mar 2022 |
| Publication status | Published - 15 Mar 2022 |