Abstract
Oxytocin induces P19 cells to differentiate into cardiomyocytes possibly through the oxytocin/oxytocin receptor system. We added oxytocin to the growth medium of P19CL6, a subline of P19, but they did not differentiate into cardiomyocytes as indicated by RT-PCR and Western blotting results. During the cardiac commitment time of P19CL6 cells, the mRNA expression levels of the oxytocin receptor were upregulated by the addition of oxytocin as well as DMSO, but an upregulation of Gata4 expression levels was only observed for the cells induced by DMSO. The in silico analysis of the upstream sequence of the oxytocin receptor predicted putative binding sites for Gata4 and Nkx2.5. These results suggest that upregulations of the oxytocin receptor and Gata4 are important for cardiomyocyte differentiation processes.
Original language | English |
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Journal | Journal of Cellular Biochemistry |
Volume | 100 |
Issue number | 3 |
Pages (from-to) | 629-641 |
Number of pages | 13 |
ISSN | 0730-2312 |
DOIs | |
Publication status | Published - 15 Feb 2007 |
Externally published | Yes |
Keywords
- Cardiac commitment
- Cardiomyocytes
- DMSO
- Oxytocin
- Oxytocin receptor
- P19CL6