TY - JOUR
T1 - Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage
AU - Bundgaard-Nielsen, Caspar
AU - Ammitzbøll, Nadia
AU - Isse, Yusuf Abdi
AU - Muqtar, Abdisalam
AU - Jensen, Ann Maria
AU - Leutscher, Peter D.C.
AU - Arenholt, Louise Thomsen Schmidt
AU - Hagstrøm, Søren
AU - Sørensen, Suzette
N1 - Copyright © 2020 Bundgaard-Nielsen, Ammitzbøll, Isse, Muqtar, Jensen, Leutscher, Arenholt, Hagstrøm and Sørensen.
PY - 2020/8/25
Y1 - 2020/8/25
N2 - Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence and risk of urinary tract infections. When comparing studies, it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition. Results: For comparison of different storage conditions, midstream urine was collected from five healthy adult female donors and analyzed by 16S rRNA gene sequencing. Samples stored at −80 and −20°C, but not 4°C, were found to be comparable to freshly handled voided urine. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in 19 healthy donors, including four women, five men, five girls, and five boys. Apart from two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals. Conclusion: The composition of voided urinary microbiota was found to be effectively maintained by freezing, but not storage at 4°C. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys. Together our study supports present methodologies that can be used in future studies investigating the urinary microbiota.
AB - Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence and risk of urinary tract infections. When comparing studies, it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition. Results: For comparison of different storage conditions, midstream urine was collected from five healthy adult female donors and analyzed by 16S rRNA gene sequencing. Samples stored at −80 and −20°C, but not 4°C, were found to be comparable to freshly handled voided urine. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in 19 healthy donors, including four women, five men, five girls, and five boys. Apart from two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals. Conclusion: The composition of voided urinary microbiota was found to be effectively maintained by freezing, but not storage at 4°C. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys. Together our study supports present methodologies that can be used in future studies investigating the urinary microbiota.
KW - bacteria
KW - DNA extraction
KW - microbiome
KW - microbiota
KW - specimen handling
KW - urine
KW - urine storage
UR - http://www.scopus.com/inward/record.url?scp=85090785353&partnerID=8YFLogxK
U2 - 10.3389/fcimb.2020.00435
DO - 10.3389/fcimb.2020.00435
M3 - Journal article
C2 - 32984068
AN - SCOPUS:85090785353
SN - 2235-2988
VL - 10
JO - Frontiers in Cellular and Infection Microbiology
JF - Frontiers in Cellular and Infection Microbiology
M1 - 435
ER -