Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

Klaus Ringsborg Westphal, Asmus Toftkær Muurmann, Iben Engell Paulsen, Kim Tanja Hejselbak Nørgaard, Marie Lund Overgaard, Sebastian Mølvang Dall, Trine Aalborg, Reinhard Wimmer, Jens Laurids Sørensen, Teis Esben Sondergaard*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.
Original languageEnglish
JournalMolecules
Volume23
Issue number2232
ISSN1420-3049
DOIs
Publication statusPublished - 2 Sept 2018

Keywords

  • Gibepyrones
  • Prolipyrone
  • Secondary metabolites
  • Polyketide synthases
  • Fusarium

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