Additional file 2 of Integrative omics reveals subtle molecular perturbations following ischemic conditioning in a porcine kidney transplant model

  • Darragh P. O'Brien (Creator)
  • Adam M. Thorne (Creator)
  • Honglei Huang (Creator)
  • Elisa Pappalardo (Creator)
  • Xuan Yao (Creator)
  • Peter Søndergaard Thyrrestrup (Creator)
  • Kristian Ravlo (Creator)
  • Niels Secher (Creator)
  • Rikke Norregaard (Creator)
  • Rutger J. Ploeg (Creator)
  • Bente Jespersen (Creator)
  • Benedikt M. Kessler (Creator)

Dataset

Description

Additional file 2: Table S1. Transcriptome sequence alignments. An average sequence alignment of 82% was achieved across all samples. Table S2. List of transcripts identified by transcriptomics. A total of 19,220 transcripts were successfully identified across groups. Table S3. Transcripts differentially expressed between RIC and Non-RIC groups. Table S4. qPCR validation on a panel of targets from discovery transcriptomics. IL1B, LTB4R, PDZD3, SLC16A3, and RASL10A were quantified by qPCR. We observed a slight increase in RIC induced SLC16A3 transcripts, but overall, none of the genes quantified reached statistically significance between RIC and non-RIC, with all having a p-value of > 0.2. Table S5. List of proteins identified by proteomics. A total of 7,546 proteins were successfully identified across groups. Table S6. Proteins differentially expressed between RIC and Non-RIC groups. Table S7. List of proteins and phosphosites identified by phosphoproteomics. A total of 3,524 phosphosites were successfully identified across groups.
Date made available2022
PublisherFigshare

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