Abstract
Interleukin 2 (IL-2) was shown to induce a small but significant increase in the level of cGMP after 20 min stimulation and a subsequent fall after 1 h in activated T lymphocytes. No change in the level of cAMP was observed. Addition of the cyclic nucleotide analogues dbcAMP or dbcGMP did not stimulate DNA synthesis. On the contrary, IL-2-induced [3H]thymidine incorporation was inhibited by these drugs. Further, the phosphodiesterase inhibitor theophylline inhibited proliferation of activated T lymphocytes. Our results indicate that neither cAMP nor cGMP act as 'second messengers' for IL-2 but support the theory that cAMP is a negative regulator of cell proliferation.
Original language | English |
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Journal | Scandinavian Journal of Immunology. Supplement |
Volume | 25 |
Issue number | 5 |
Pages (from-to) | 527-31 |
Number of pages | 5 |
ISSN | 0300-9475 |
Publication status | Published - May 1987 |
Externally published | Yes |
Keywords
- Bucladesine
- Dibutyryl Cyclic GMP
- Humans
- Interleukin-2
- Lymphocyte Activation
- Nucleotides, Cyclic
- Receptors, Immunologic
- Receptors, Interleukin-2
- T-Lymphocytes
- Theophylline