Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study

Rasmus Hagn-Meincke; Phil A. Hart; Dana K. Andersen; Santhi S. Vege; Evan L. Fogel; Jose Serrano; Melena D. Bellin; Mark D. Topazian; Darwin L. Conwell; Liang Li; Stephen K. Van Den Eeden; Asbjørn M. Drewes; Stephen J. Pandol; Chris E. Forsmark; William E. Fisher; Dhiraj Yadav; Søren S. Olesen; Walter G. Park; Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC)

doi:10.1097/MEG.0000000000002691

Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study

Rasmus Hagn-Meincke, Phil A. Hart, Dana K. Andersen, Santhi S. Vege, Evan L. Fogel, Jose Serrano, Melena D. Bellin, Mark D. Topazian, Darwin L. Conwell, Liang Li, Stephen K. Van Den Eeden, Asbjørn M. Drewes, Stephen J. Pandol, Chris E. Forsmark, William E. Fisher, Dhiraj Yadav, Søren S. Olesen, Walter G. Park^*, Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC)

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

1 Citation (Scopus)

Abstract

OBJECTIVE: This pilot study seeks to identify serum immune signatures across clinical stages of patients with chronic pancreatitis (CP).

METHODS: We performed a cross-sectional analysis of prospectively collected serum samples from the PROspective Evaluation of Chronic Pancreatitis for EpidEmiologic and Translation StuDies-study. CP subjects were categorised into three clinical stages based on the presence/absence of metabolic complications: (1) CP with no diabetes and exocrine pancreatic dysfunction (EPD), (2) CP with either diabetes or EPD, and (3) CP with diabetes and EPD. Blinded samples were analysed using an 80-plex Luminex assay of cytokines/chemokines/adhesion molecules. Group and pairwise comparisons were performed to characterise immune signatures across CP subgroups.

RESULTS: A total of 135 CP subjects (evenly distributed between clinical stages) and 50 controls were studied. Interleukin-6 (IL-6), interleukin-8 (IL-8), and soluble intercellular adhesion molecule 1 (sICAM-1) were significantly elevated in CP subjects compared to controls. The levels of IL-6 and IL-8 increased with advancing disease stages, with the highest levels observed in CP with diabetes and EPD (clinical stage 3). Furthermore, hepatocyte growth factor and macrophage-derived chemokine were significantly increased in clinical stage 3 compared to controls.

CONCLUSION: Our study reveals a progressive elevation in pro-inflammatory cytokines and chemokines with advancing clinical stages of CP. These findings indicate potential targets for the development of disease-modifying interventions.

Original language	English
Journal	European Journal of Gastroenterology and Hepatology
Volume	36
Issue number	2
Pages (from-to)	177-183
Number of pages	7
ISSN	0954-691X
DOIs	https://doi.org/10.1097/MEG.0000000000002691
Publication status	Published - Feb 2024

Bibliographical note

Keywords

Chemokines
Cross-Sectional Studies
Cytokines
Diabetes Mellitus
Humans
Interleukin-6
Interleukin-8/analysis
Pancreatitis, Chronic/diagnosis
Pilot Projects
chronic pancreatitis
immune signatures
metabolic complications

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Hagn-Meincke, R., Hart, P. A., Andersen, D. K., Vege, S. S., Fogel, E. L., Serrano, J., Bellin, M. D., Topazian, M. D., Conwell, D. L., Li, L., Van Den Eeden, S. K., Drewes, A. M., Pandol, S. J., Forsmark, C. E., Fisher, W. E., Yadav, D., Olesen, S. S., Park, W. G., & Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC) (2024). Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study. European Journal of Gastroenterology and Hepatology, 36(2), 177-183. https://doi.org/10.1097/MEG.0000000000002691

@article{76506bcfb64d41738cabd872e7d9aaeb,

title = "Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study",

abstract = "OBJECTIVE: This pilot study seeks to identify serum immune signatures across clinical stages of patients with chronic pancreatitis (CP).METHODS: We performed a cross-sectional analysis of prospectively collected serum samples from the PROspective Evaluation of Chronic Pancreatitis for EpidEmiologic and Translation StuDies-study. CP subjects were categorised into three clinical stages based on the presence/absence of metabolic complications: (1) CP with no diabetes and exocrine pancreatic dysfunction (EPD), (2) CP with either diabetes or EPD, and (3) CP with diabetes and EPD. Blinded samples were analysed using an 80-plex Luminex assay of cytokines/chemokines/adhesion molecules. Group and pairwise comparisons were performed to characterise immune signatures across CP subgroups.RESULTS: A total of 135 CP subjects (evenly distributed between clinical stages) and 50 controls were studied. Interleukin-6 (IL-6), interleukin-8 (IL-8), and soluble intercellular adhesion molecule 1 (sICAM-1) were significantly elevated in CP subjects compared to controls. The levels of IL-6 and IL-8 increased with advancing disease stages, with the highest levels observed in CP with diabetes and EPD (clinical stage 3). Furthermore, hepatocyte growth factor and macrophage-derived chemokine were significantly increased in clinical stage 3 compared to controls.CONCLUSION: Our study reveals a progressive elevation in pro-inflammatory cytokines and chemokines with advancing clinical stages of CP. These findings indicate potential targets for the development of disease-modifying interventions.",

keywords = "Chemokines, Cross-Sectional Studies, Cytokines, Diabetes Mellitus, Humans, Interleukin-6, Interleukin-8/analysis, Pancreatitis, Chronic/diagnosis, Pilot Projects, chronic pancreatitis, immune signatures, metabolic complications",

author = "Rasmus Hagn-Meincke and Hart, {Phil A.} and Andersen, {Dana K.} and Vege, {Santhi S.} and Fogel, {Evan L.} and Jose Serrano and Bellin, {Melena D.} and Topazian, {Mark D.} and Conwell, {Darwin L.} and Liang Li and {Van Den Eeden}, {Stephen K.} and Drewes, {Asbj{\o}rn M.} and Pandol, {Stephen J.} and Forsmark, {Chris E.} and Fisher, {William E.} and Dhiraj Yadav and Olesen, {S{\o}ren S.} and Park, {Walter G.} and {Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC)}",

year = "2024",

month = feb,

doi = "10.1097/MEG.0000000000002691",

language = "English",

volume = "36",

pages = "177--183",

journal = "European Journal of Gastroenterology and Hepatology",

issn = "0954-691X",

publisher = "Lippincott Williams & Wilkins, Ltd.",

number = "2",

}

Hagn-Meincke, R, Hart, PA, Andersen, DK, Vege, SS, Fogel, EL, Serrano, J, Bellin, MD, Topazian, MD, Conwell, DL, Li, L, Van Den Eeden, SK, Drewes, AM, Pandol, SJ, Forsmark, CE, Fisher, WE, Yadav, D, Olesen, SS, Park, WG & Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC) 2024, 'Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study', European Journal of Gastroenterology and Hepatology, vol. 36, no. 2, pp. 177-183. https://doi.org/10.1097/MEG.0000000000002691

TY - JOUR

T1 - Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study

AU - Hagn-Meincke, Rasmus

AU - Hart, Phil A.

AU - Andersen, Dana K.

AU - Vege, Santhi S.

AU - Fogel, Evan L.

AU - Serrano, Jose

AU - Bellin, Melena D.

AU - Topazian, Mark D.

AU - Conwell, Darwin L.

AU - Li, Liang

AU - Van Den Eeden, Stephen K.

AU - Drewes, Asbjørn M.

AU - Pandol, Stephen J.

AU - Forsmark, Chris E.

AU - Fisher, William E.

AU - Yadav, Dhiraj

AU - Olesen, Søren S.

AU - Park, Walter G.

AU - Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC)

PY - 2024/2

Y1 - 2024/2

N2 - OBJECTIVE: This pilot study seeks to identify serum immune signatures across clinical stages of patients with chronic pancreatitis (CP).METHODS: We performed a cross-sectional analysis of prospectively collected serum samples from the PROspective Evaluation of Chronic Pancreatitis for EpidEmiologic and Translation StuDies-study. CP subjects were categorised into three clinical stages based on the presence/absence of metabolic complications: (1) CP with no diabetes and exocrine pancreatic dysfunction (EPD), (2) CP with either diabetes or EPD, and (3) CP with diabetes and EPD. Blinded samples were analysed using an 80-plex Luminex assay of cytokines/chemokines/adhesion molecules. Group and pairwise comparisons were performed to characterise immune signatures across CP subgroups.RESULTS: A total of 135 CP subjects (evenly distributed between clinical stages) and 50 controls were studied. Interleukin-6 (IL-6), interleukin-8 (IL-8), and soluble intercellular adhesion molecule 1 (sICAM-1) were significantly elevated in CP subjects compared to controls. The levels of IL-6 and IL-8 increased with advancing disease stages, with the highest levels observed in CP with diabetes and EPD (clinical stage 3). Furthermore, hepatocyte growth factor and macrophage-derived chemokine were significantly increased in clinical stage 3 compared to controls.CONCLUSION: Our study reveals a progressive elevation in pro-inflammatory cytokines and chemokines with advancing clinical stages of CP. These findings indicate potential targets for the development of disease-modifying interventions.

AB - OBJECTIVE: This pilot study seeks to identify serum immune signatures across clinical stages of patients with chronic pancreatitis (CP).METHODS: We performed a cross-sectional analysis of prospectively collected serum samples from the PROspective Evaluation of Chronic Pancreatitis for EpidEmiologic and Translation StuDies-study. CP subjects were categorised into three clinical stages based on the presence/absence of metabolic complications: (1) CP with no diabetes and exocrine pancreatic dysfunction (EPD), (2) CP with either diabetes or EPD, and (3) CP with diabetes and EPD. Blinded samples were analysed using an 80-plex Luminex assay of cytokines/chemokines/adhesion molecules. Group and pairwise comparisons were performed to characterise immune signatures across CP subgroups.RESULTS: A total of 135 CP subjects (evenly distributed between clinical stages) and 50 controls were studied. Interleukin-6 (IL-6), interleukin-8 (IL-8), and soluble intercellular adhesion molecule 1 (sICAM-1) were significantly elevated in CP subjects compared to controls. The levels of IL-6 and IL-8 increased with advancing disease stages, with the highest levels observed in CP with diabetes and EPD (clinical stage 3). Furthermore, hepatocyte growth factor and macrophage-derived chemokine were significantly increased in clinical stage 3 compared to controls.CONCLUSION: Our study reveals a progressive elevation in pro-inflammatory cytokines and chemokines with advancing clinical stages of CP. These findings indicate potential targets for the development of disease-modifying interventions.

KW - Chemokines

KW - Cross-Sectional Studies

KW - Cytokines

KW - Diabetes Mellitus

KW - Humans

KW - Interleukin-6

KW - Interleukin-8/analysis

KW - Pancreatitis, Chronic/diagnosis

KW - Pilot Projects

KW - chronic pancreatitis

KW - immune signatures

KW - metabolic complications

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U2 - 10.1097/MEG.0000000000002691

DO - 10.1097/MEG.0000000000002691

M3 - Journal article

C2 - 38047728

SN - 0954-691X

VL - 36

SP - 177

EP - 183

JO - European Journal of Gastroenterology and Hepatology

JF - European Journal of Gastroenterology and Hepatology

IS - 2

ER -

Circulating immune signatures across clinical stages of chronic pancreatitis: a pilot study

Abstract

Bibliographical note

Keywords

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