Abstract
Elucidating genes that affect life span or that can be used as biomarkers for ageing has received attention in diverse studies in recent years. Using model organisms and various approaches several genes have been linked to the longevity phenotype. For Drosophila melanogaster those studies have usually focussed on one sex and on flies originating from one genetic background, and results from different studies often do not overlap. Using D. melanogaster selected for increased longevity we aimed to find robust longevity related genes by examining gene expression in both sexes of flies originating from different genetic backgrounds. Further, we compared expression changes across three ages, when flies were young, middle aged or old, to examine how candidate gene expression changes with the onset of ageing. We selected 10 genes based on their expression differences in prior microarray studies. For about 50% of these we confirmed their potential as a candidate longevity gene. We found one robust candidate gene for longevity, CG32638. Three other genes, CG8934, mRpS10 and Spn43Ad, showed a tendency to be involved in life span determination in both backgrounds tested.
Original language | English |
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Journal | Journal of Insect Physiology |
Volume | 59 |
Issue number | 3 |
Pages (from-to) | 255-62 |
Number of pages | 8 |
ISSN | 0022-1910 |
DOIs | |
Publication status | Published - Mar 2013 |
Externally published | Yes |
Bibliographical note
Copyright © 2013 Elsevier Ltd. All rights reserved.Keywords
- Animals
- Drosophila Proteins/genetics
- Drosophila melanogaster/genetics
- Female
- Gene Expression Regulation, Developmental
- Genetic Markers
- Longevity
- Male
- Real-Time Polymerase Chain Reaction