Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species

Anna M. Lewinska; Jakob B. Nielsen; Ruut H. Peuhkuri; Carsten Rode; Birgitte Andersen

Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species

Anna M. Lewinska^*, Jakob B. Nielsen, Ruut H. Peuhkuri, Carsten Rode, Birgitte Andersen

^*Corresponding author for this work

Research output: Contribution to book/anthology/report/conference proceeding › Article in proceeding › Research › peer-review

Abstract

Detection and identification of indoor fungi in water-damaged buildings is crucial for preventi and control of fungal growth. This study focuses on a molecular method called DNA barcoding. evaluates commonly used sequences in DNA barcoding for fungal species identification Chaetomium and Stachybotrys. The existing DNA barcodes: ITS, SSU, LSU, B-TUB, CMD, RP and TEF-1α do not give satisfying species resolution to be considered as DNA barcodes for the two genera. Therefore, novel barcodes for them are needed. Barcode potentials, such as HOG1 a NAHA, were identified using bioinformatics and are being evaluated in laboratory.

Original language	English
Title of host publication	Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate
Number of pages	8
Publisher	International Society of Indoor Air Quality and Climate
Publication date	2014
Pages	281-288
Publication status	Published - 2014
Event	13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014: The 13th International Conference on Indoor Air Quality and Climate - Hong Kong, Hong Kong Duration: 7 Jul 2014 → 12 Jul 2014

Conference

Conference	13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014
Country/Territory	Hong Kong
City	Hong Kong
Period	07/07/2014 → 12/07/2014

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@inproceedings{5c7f8115454c4e75bfe7632c06aca073,

title = "Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species",

abstract = "Detection and identification of indoor fungi in water-damaged buildings is crucial for preventi and control of fungal growth. This study focuses on a molecular method called DNA barcoding. evaluates commonly used sequences in DNA barcoding for fungal species identification Chaetomium and Stachybotrys. The existing DNA barcodes: ITS, SSU, LSU, B-TUB, CMD, RP and TEF-1α do not give satisfying species resolution to be considered as DNA barcodes for the two genera. Therefore, novel barcodes for them are needed. Barcode potentials, such as HOG1 a NAHA, were identified using bioinformatics and are being evaluated in laboratory.",

author = "Lewinska, {Anna M.} and Nielsen, {Jakob B.} and Peuhkuri, {Ruut H.} and Carsten Rode and Birgitte Andersen",

year = "2014",

language = "English",

pages = "281--288",

booktitle = "Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate",

publisher = "International Society of Indoor Air Quality and Climate",

note = "13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014 ; Conference date: 07-07-2014 Through 12-07-2014",

}

Lewinska, AM, Nielsen, JB, Peuhkuri, RH, Rode, C & Andersen, B 2014, Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species. in Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate. International Society of Indoor Air Quality and Climate, pp. 281-288, 13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014, Hong Kong, Hong Kong, 07/07/2014.

Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species. / Lewinska, Anna M.; Nielsen, Jakob B.; Peuhkuri, Ruut H. et al.
Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate. International Society of Indoor Air Quality and Climate, 2014. p. 281-288.

Research output: Contribution to book/anthology/report/conference proceeding › Article in proceeding › Research › peer-review

TY - GEN

T1 - Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species

AU - Lewinska, Anna M.

AU - Nielsen, Jakob B.

AU - Peuhkuri, Ruut H.

AU - Rode, Carsten

AU - Andersen, Birgitte

PY - 2014

Y1 - 2014

N2 - Detection and identification of indoor fungi in water-damaged buildings is crucial for preventi and control of fungal growth. This study focuses on a molecular method called DNA barcoding. evaluates commonly used sequences in DNA barcoding for fungal species identification Chaetomium and Stachybotrys. The existing DNA barcodes: ITS, SSU, LSU, B-TUB, CMD, RP and TEF-1α do not give satisfying species resolution to be considered as DNA barcodes for the two genera. Therefore, novel barcodes for them are needed. Barcode potentials, such as HOG1 a NAHA, were identified using bioinformatics and are being evaluated in laboratory.

AB - Detection and identification of indoor fungi in water-damaged buildings is crucial for preventi and control of fungal growth. This study focuses on a molecular method called DNA barcoding. evaluates commonly used sequences in DNA barcoding for fungal species identification Chaetomium and Stachybotrys. The existing DNA barcodes: ITS, SSU, LSU, B-TUB, CMD, RP and TEF-1α do not give satisfying species resolution to be considered as DNA barcodes for the two genera. Therefore, novel barcodes for them are needed. Barcode potentials, such as HOG1 a NAHA, were identified using bioinformatics and are being evaluated in laboratory.

UR - http://www.scopus.com/inward/record.url?scp=84924703638&partnerID=8YFLogxK

M3 - Article in proceeding

AN - SCOPUS:84924703638

SP - 281

EP - 288

BT - Indoor Air 2014 - 13th International Conference on Indoor Air Quality and Climate

PB - International Society of Indoor Air Quality and Climate

T2 - 13th International Conference on Indoor Air Quality and Climate, Indoor Air 2014

Y2 - 7 July 2014 through 12 July 2014

ER -

Novel DNA barcodes for detection, idenfication and tracking of stachybotrys and chaetomium species

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