Supported silver clusters as nanoplasmonic transducers for protein sensing

Peter Fojan, Muhammad Hanif, Stephen Bartling, Hannes Hartmann, Ingo Barke, Vladimir Popok

    Research output: Contribution to journalJournal articleResearchpeer-review

    7 Citations (Scopus)
    350 Downloads (Pure)

    Abstract

    Transducers for optical sensing of proteins are prepared using cluster beam deposition on quartz substrates. Surface plasmon resonance phenomenon of the supported silver clusters is used for the detection. It is shown that surface immobilisation procedure providing adhesion of the silver clusters to quartz and functionalization of cluster surfaces for antibody coupling are the key issues for cluster stability and protein detection. Focus was put on these tasks and the processes have been optimised. In particular, conditions for coupling of the antibodies to the clusters are developed providing an enhancement of the plasmon absorption band used for the detection. Atomic force microscopy study allows to suggest that immobilisation of antibodies on silver clusters has been achieved, thus giving a possibility to incubate and detect an antigen of interest. Hence, by applying the developed preparation stages and protein immobilisation scheme the sensing of protein of interest can be assured using a relatively simple optical spectroscopy method.
    Original languageEnglish
    JournalSensors and Actuators B: Chemical
    Volume212
    Pages (from-to)377-381
    Number of pages7
    ISSN0925-4005
    DOIs
    Publication statusPublished - Jun 2015

    Cite this

    Fojan, Peter ; Hanif, Muhammad ; Bartling, Stephen ; Hartmann, Hannes ; Barke, Ingo ; Popok, Vladimir. / Supported silver clusters as nanoplasmonic transducers for protein sensing. In: Sensors and Actuators B: Chemical. 2015 ; Vol. 212. pp. 377-381.
    @article{1a4f2838e8784599b67c4081a74be4d5,
    title = "Supported silver clusters as nanoplasmonic transducers for protein sensing",
    abstract = "Transducers for optical sensing of proteins are prepared using cluster beam deposition on quartz substrates. Surface plasmon resonance phenomenon of the supported silver clusters is used for the detection. It is shown that surface immobilisation procedure providing adhesion of the silver clusters to quartz and functionalization of cluster surfaces for antibody coupling are the key issues for cluster stability and protein detection. Focus was put on these tasks and the processes have been optimised. In particular, conditions for coupling of the antibodies to the clusters are developed providing an enhancement of the plasmon absorption band used for the detection. Atomic force microscopy study allows to suggest that immobilisation of antibodies on silver clusters has been achieved, thus giving a possibility to incubate and detect an antigen of interest. Hence, by applying the developed preparation stages and protein immobilisation scheme the sensing of protein of interest can be assured using a relatively simple optical spectroscopy method.",
    keywords = "cluster beam deposition, metal nanoparticles , localised surface plasmon resonance , proteins , transducers for optical sensing",
    author = "Peter Fojan and Muhammad Hanif and Stephen Bartling and Hannes Hartmann and Ingo Barke and Vladimir Popok",
    year = "2015",
    month = "6",
    doi = "10.1016/j.snb.2015.01.131",
    language = "English",
    volume = "212",
    pages = "377--381",
    journal = "Sensors and Actuators B: Chemical",
    issn = "0925-4005",
    publisher = "Elsevier",

    }

    Supported silver clusters as nanoplasmonic transducers for protein sensing. / Fojan, Peter; Hanif, Muhammad; Bartling, Stephen; Hartmann, Hannes; Barke, Ingo; Popok, Vladimir.

    In: Sensors and Actuators B: Chemical, Vol. 212, 06.2015, p. 377-381.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Supported silver clusters as nanoplasmonic transducers for protein sensing

    AU - Fojan, Peter

    AU - Hanif, Muhammad

    AU - Bartling, Stephen

    AU - Hartmann, Hannes

    AU - Barke, Ingo

    AU - Popok, Vladimir

    PY - 2015/6

    Y1 - 2015/6

    N2 - Transducers for optical sensing of proteins are prepared using cluster beam deposition on quartz substrates. Surface plasmon resonance phenomenon of the supported silver clusters is used for the detection. It is shown that surface immobilisation procedure providing adhesion of the silver clusters to quartz and functionalization of cluster surfaces for antibody coupling are the key issues for cluster stability and protein detection. Focus was put on these tasks and the processes have been optimised. In particular, conditions for coupling of the antibodies to the clusters are developed providing an enhancement of the plasmon absorption band used for the detection. Atomic force microscopy study allows to suggest that immobilisation of antibodies on silver clusters has been achieved, thus giving a possibility to incubate and detect an antigen of interest. Hence, by applying the developed preparation stages and protein immobilisation scheme the sensing of protein of interest can be assured using a relatively simple optical spectroscopy method.

    AB - Transducers for optical sensing of proteins are prepared using cluster beam deposition on quartz substrates. Surface plasmon resonance phenomenon of the supported silver clusters is used for the detection. It is shown that surface immobilisation procedure providing adhesion of the silver clusters to quartz and functionalization of cluster surfaces for antibody coupling are the key issues for cluster stability and protein detection. Focus was put on these tasks and the processes have been optimised. In particular, conditions for coupling of the antibodies to the clusters are developed providing an enhancement of the plasmon absorption band used for the detection. Atomic force microscopy study allows to suggest that immobilisation of antibodies on silver clusters has been achieved, thus giving a possibility to incubate and detect an antigen of interest. Hence, by applying the developed preparation stages and protein immobilisation scheme the sensing of protein of interest can be assured using a relatively simple optical spectroscopy method.

    KW - cluster beam deposition

    KW - metal nanoparticles

    KW - localised surface plasmon resonance

    KW - proteins

    KW - transducers for optical sensing

    U2 - 10.1016/j.snb.2015.01.131

    DO - 10.1016/j.snb.2015.01.131

    M3 - Journal article

    VL - 212

    SP - 377

    EP - 381

    JO - Sensors and Actuators B: Chemical

    JF - Sensors and Actuators B: Chemical

    SN - 0925-4005

    ER -