Supported silver clusters as nanoplasmonic transducers for protein sensing

Peter Fojan, Muhammad Hanif, Stephen Bartling, Hannes Hartmann, Ingo Barke, Vladimir Popok

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    Abstract

    Transducers for optical sensing of proteins are prepared using cluster beam deposition on quartz substrates. Surface plasmon resonance phenomenon of the supported silver clusters is used for the detection. It is shown that surface immobilisation procedure providing adhesion of the silver clusters to quartz and functionalization of cluster surfaces for antibody coupling are the key issues for cluster stability and protein detection. Focus was put on these tasks and the processes have been optimised. In particular, conditions for coupling of the antibodies to the clusters are developed providing an enhancement of the plasmon absorption band used for the detection. Atomic force microscopy study allows to suggest that immobilisation of antibodies on silver clusters has been achieved, thus giving a possibility to incubate and detect an antigen of interest. Hence, by applying the developed preparation stages and protein immobilisation scheme the sensing of protein of interest can be assured using a relatively simple optical spectroscopy method.
    Original languageEnglish
    JournalSensors and Actuators B: Chemical
    Volume212
    Pages (from-to)377-381
    Number of pages7
    ISSN0925-4005
    DOIs
    Publication statusPublished - Jun 2015

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