TY - JOUR
T1 - Children and adolescents with attention deficit hyperactivity disorder and autism spectrum disorder share distinct microbiota compositions
AU - Bundgaard-Nielsen, Caspar
AU - Lauritsen, Marlene Briciet
AU - Knudsen, Julie Kristine
AU - Rold, Louise Søndergaard
AU - Larsen, Margit Hørup
AU - Hindersson, Peter
AU - Villadsen, Annemarie Brusen
AU - Leutscher, Peter D. C.
AU - Hagstrøm, Søren
AU - Nyegaard, Mette
AU - Sørensen, Suzette
PY - 2023
Y1 - 2023
N2 - An association has been suggested between altered gut microbiota, and attention deficit hyperactivity disorder (ADHD), and autism spectrum disorder (ASD), respectively. Thus, we analyzed the gut microbiota composition in children and adolescents with or without these disorders and evaluated the systemic effects of these bacteria. We recruited study participants diagnosed with ADHD, ASD, and comorbid ADHD/ASD, while the control groups consisted both of siblings and non-related children. The gut microbiota was analyzed by 16S rRNA gene sequencing of the V4 region, while the concentration of lipopolysaccharide-binding protein (LBP), cytokines, and other signaling molecules were measured in plasma. Importantly the gut microbiota compositions of cases with ADHD and ASD were highly similar for both alpha- and beta-diversity while differing from that of non-related controls. Furthermore, a subset of ADHD and ASD cases had an increased LBP concentration compared to non-affected children, which was positively correlated with interleukin (IL)-8, 12, and 13. These observations indicate disruption of the intestinal barrier and immune dysregulation among the subset of children with ADHD or ASD.
AB - An association has been suggested between altered gut microbiota, and attention deficit hyperactivity disorder (ADHD), and autism spectrum disorder (ASD), respectively. Thus, we analyzed the gut microbiota composition in children and adolescents with or without these disorders and evaluated the systemic effects of these bacteria. We recruited study participants diagnosed with ADHD, ASD, and comorbid ADHD/ASD, while the control groups consisted both of siblings and non-related children. The gut microbiota was analyzed by 16S rRNA gene sequencing of the V4 region, while the concentration of lipopolysaccharide-binding protein (LBP), cytokines, and other signaling molecules were measured in plasma. Importantly the gut microbiota compositions of cases with ADHD and ASD were highly similar for both alpha- and beta-diversity while differing from that of non-related controls. Furthermore, a subset of ADHD and ASD cases had an increased LBP concentration compared to non-affected children, which was positively correlated with interleukin (IL)-8, 12, and 13. These observations indicate disruption of the intestinal barrier and immune dysregulation among the subset of children with ADHD or ASD.
KW - Adolescent
KW - Attention Deficit Disorder with Hyperactivity
KW - Autism Spectrum Disorder
KW - Child
KW - Gastrointestinal Microbiome/genetics
KW - Humans
KW - Microbiota
KW - RNA, Ribosomal, 16S/genetics
KW - microbiota
KW - attention deficit hyperactivity disorder
KW - microbiome
KW - neurodevelopmental disorders
KW - Autism spectrum disorder
KW - inflammation
KW - gut-brain axis
UR - http://www.scopus.com/inward/record.url?scp=85159760599&partnerID=8YFLogxK
U2 - 10.1080/19490976.2023.2211923
DO - 10.1080/19490976.2023.2211923
M3 - Journal article
C2 - 37199526
SN - 1949-0976
VL - 15
JO - Gut Microbes
JF - Gut Microbes
IS - 1
M1 - 2211923
ER -