Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters

Morten Simonsen Dueholm; Simon Knutsson; Vibeke Rudkjøbing Jørgensen; Marta Anna Nierychlo; Jannie Munk Kristensen; Francesca Petriglieri; Erika Yashiro; Søren Michael Karst; Mads Albertsen; Per Halkjær Nielsen

Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters

Morten Simonsen Dueholm, Simon Knutsson, Vibeke Rudkjøbing Jørgensen, Marta Anna Nierychlo, Jannie Munk Kristensen, Francesca Petriglieri, Erika Yashiro, Søren Michael Karst, Mads Albertsen, Per Halkjær Nielsen

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Forskning

Abstract

We have recently developed a method that can produce millions of high-quality, full-length 16S rRNA sequences from any environment. Here we used this method to generate 412,174 of such sequences from anaerobic digesters located at Danish wastewater treatment plants. The sequences were used to create the first comprehensive anaerobic digester specific 16S rRNA reference database containing 9,174 unique full-length 16S rRNA sequences. Phylogenetic analyses of the database revealed many novel microbes, which highlights large gaps in our current knowledge regarding the microorganism involved in the anaerobic digestion process. The reference database now allows us to assign provisional names to these organisms and start to connect them with their ecological roles in the AD process.

Originalsprog	Engelsk
Publikationsdato	24 jun. 2019
Status	Udgivet - 24 jun. 2019
Begivenhed	16th IWA World Conference on Anaerobic digestion - Delft, Holland Varighed: 23 jun. 2019 → 27 jun. 2019 https://www.ad16conference.com/

Konference

Konference	16th IWA World Conference on Anaerobic digestion
Land/Område	Holland
By	Delft
Periode	23/06/2019 → 27/06/2019
Internetadresse	https://www.ad16conference.com/

Adgang til dokumentet

https://www.ad16conference.com/programme/assets/uploads/2019/04/28792-.pdf

AUB Link

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Citationsformater

Dueholm, M. S., Knutsson, S., Jørgensen, V. R., Nierychlo, M. A., Kristensen, J. M., Petriglieri, F., Yashiro, E., Karst, S. M., Albertsen, M., & Nielsen, P. H. (2019). Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters. Abstract fra 16th IWA World Conference on Anaerobic digestion, Delft, Holland. https://www.ad16conference.com/programme/assets/uploads/2019/04/28792-.pdf

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title = "Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters",

abstract = "We have recently developed a method that can produce millions of high-quality, full-length 16S rRNA sequences from any environment. Here we used this method to generate 412,174 of such sequences from anaerobic digesters located at Danish wastewater treatment plants. The sequences were used to create the first comprehensive anaerobic digester specific 16S rRNA reference database containing 9,174 unique full-length 16S rRNA sequences. Phylogenetic analyses of the database revealed many novel microbes, which highlights large gaps in our current knowledge regarding the microorganism involved in the anaerobic digestion process. The reference database now allows us to assign provisional names to these organisms and start to connect them with their ecological roles in the AD process.",

author = "Dueholm, {Morten Simonsen} and Simon Knutsson and J{\o}rgensen, {Vibeke Rudkj{\o}bing} and Nierychlo, {Marta Anna} and Kristensen, {Jannie Munk} and Francesca Petriglieri and Erika Yashiro and Karst, {S{\o}ren Michael} and Mads Albertsen and Nielsen, {Per Halkj{\ae}r}",

year = "2019",

month = jun,

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note = "16th IWA World Conference on Anaerobic digestion ; Conference date: 23-06-2019 Through 27-06-2019",

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Dueholm, MS, Knutsson, S, Jørgensen, VR, Nierychlo, MA , Kristensen, JM , Petriglieri, F, Yashiro, E, Karst, SM, Albertsen, M & Nielsen, PH 2019, 'Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters', 16th IWA World Conference on Anaerobic digestion, Delft, Holland, 23/06/2019 - 27/06/2019. <https://www.ad16conference.com/programme/assets/uploads/2019/04/28792-.pdf>

Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters. / Dueholm, Morten Simonsen; Knutsson, Simon; Jørgensen, Vibeke Rudkjøbing et al.
2019. Abstract fra 16th IWA World Conference on Anaerobic digestion, Delft, Holland.

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Forskning

TY - ABST

T1 - Comprehensive ecosystem-specific 16S rRNA database illuminates the microbial dark matter in anaerobic digesters

AU - Dueholm, Morten Simonsen

AU - Knutsson, Simon

AU - Jørgensen, Vibeke Rudkjøbing

AU - Nierychlo, Marta Anna

AU - Kristensen, Jannie Munk

AU - Petriglieri, Francesca

AU - Yashiro, Erika

AU - Karst, Søren Michael

AU - Albertsen, Mads

AU - Nielsen, Per Halkjær

PY - 2019/6/24

Y1 - 2019/6/24

N2 - We have recently developed a method that can produce millions of high-quality, full-length 16S rRNA sequences from any environment. Here we used this method to generate 412,174 of such sequences from anaerobic digesters located at Danish wastewater treatment plants. The sequences were used to create the first comprehensive anaerobic digester specific 16S rRNA reference database containing 9,174 unique full-length 16S rRNA sequences. Phylogenetic analyses of the database revealed many novel microbes, which highlights large gaps in our current knowledge regarding the microorganism involved in the anaerobic digestion process. The reference database now allows us to assign provisional names to these organisms and start to connect them with their ecological roles in the AD process.

AB - We have recently developed a method that can produce millions of high-quality, full-length 16S rRNA sequences from any environment. Here we used this method to generate 412,174 of such sequences from anaerobic digesters located at Danish wastewater treatment plants. The sequences were used to create the first comprehensive anaerobic digester specific 16S rRNA reference database containing 9,174 unique full-length 16S rRNA sequences. Phylogenetic analyses of the database revealed many novel microbes, which highlights large gaps in our current knowledge regarding the microorganism involved in the anaerobic digestion process. The reference database now allows us to assign provisional names to these organisms and start to connect them with their ecological roles in the AD process.

M3 - Conference abstract for conference

T2 - 16th IWA World Conference on Anaerobic digestion

Y2 - 23 June 2019 through 27 June 2019

ER -