TY - JOUR
T1 - Method for a homogeneous distribution of pollens in an environmental exposure chamber
AU - Kenney, P.
AU - Bønløkke, J.
AU - Hilberg, O.
AU - Ravn, P.
AU - Schlünssen, V.
AU - Sigsgaard, T.
N1 - Publisher Copyright:
© 2016 John Wiley & Sons Ltd
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Background: A variety of different environmental exposure chambers (EECs) have been used to evaluate treatments for allergic rhinitis. Objective: To describe and test a system for a homogenous distribution of grass pollen, Phleum Pratense, in an EEC to be used for controlled pollen exposure studies in allergic participants. Methods: A chamber made of stainless steel with completely rounded corners, seating four individuals at a time, was used. Room pressure, temperature, humidity and the air change rate were kept constant throughout the study period. A rotating pipette dispensed a uniform supply of pollen into a turntable's v-shaped grooves. A stainless steel capillary tube sucked the pollens into a venturi throat at which time the pollens were mixed with a high-pressure airstream of compressed high-efficiency particulate arrestance filtered air and then transported to a spreading plate inside the EEC. To achieve uniform concentrations in the EEC, the turntable's rotating speed was continuously adjusted using information from video-coupled feedback and feed forward mechanisms. Pollen levels were detected using standard volumetric air samplers and laser particle counters. The target pollen exposure level was 1000 pollens/m3. Twenty-one participants were exposed to pollens in the EEC twice for 210 min. Participants evaluated their symptoms every 30 min using a total nasal symptom score (TNSS) consisting of blocked nose, runny nose, nasal itching and sneezing. Results: Across fifteen study days, the average pollen level was 982 pollens/m3 (SD, 102 pollens/m3). On average, participants experienced a 10% difference in overall pollen levels between their two visits to the EEC. The mean TNSSs rose throughout the exposure period, with a low at baseline of 0.43 (SD, 0.68) to a high of 4.71 (SD, 2.43) just before exiting the EEC. Conclusion and Clinical Relevance: This EEC provides a reproducible, precise and homogenous distribution of pollens making it suitable for single-centre allergy clinical trials.
AB - Background: A variety of different environmental exposure chambers (EECs) have been used to evaluate treatments for allergic rhinitis. Objective: To describe and test a system for a homogenous distribution of grass pollen, Phleum Pratense, in an EEC to be used for controlled pollen exposure studies in allergic participants. Methods: A chamber made of stainless steel with completely rounded corners, seating four individuals at a time, was used. Room pressure, temperature, humidity and the air change rate were kept constant throughout the study period. A rotating pipette dispensed a uniform supply of pollen into a turntable's v-shaped grooves. A stainless steel capillary tube sucked the pollens into a venturi throat at which time the pollens were mixed with a high-pressure airstream of compressed high-efficiency particulate arrestance filtered air and then transported to a spreading plate inside the EEC. To achieve uniform concentrations in the EEC, the turntable's rotating speed was continuously adjusted using information from video-coupled feedback and feed forward mechanisms. Pollen levels were detected using standard volumetric air samplers and laser particle counters. The target pollen exposure level was 1000 pollens/m3. Twenty-one participants were exposed to pollens in the EEC twice for 210 min. Participants evaluated their symptoms every 30 min using a total nasal symptom score (TNSS) consisting of blocked nose, runny nose, nasal itching and sneezing. Results: Across fifteen study days, the average pollen level was 982 pollens/m3 (SD, 102 pollens/m3). On average, participants experienced a 10% difference in overall pollen levels between their two visits to the EEC. The mean TNSSs rose throughout the exposure period, with a low at baseline of 0.43 (SD, 0.68) to a high of 4.71 (SD, 2.43) just before exiting the EEC. Conclusion and Clinical Relevance: This EEC provides a reproducible, precise and homogenous distribution of pollens making it suitable for single-centre allergy clinical trials.
KW - Allergen exposure chamber
KW - Allergic Rhinitis
KW - Clinical trial
KW - Controlled
KW - Controlled allergen challenge
KW - Environmental Exposure Chamber
KW - grass pollen
KW - Pollen exposure
UR - http://www.scopus.com/inward/record.url?scp=84984596880&partnerID=8YFLogxK
U2 - 10.1111/cea.12747
DO - 10.1111/cea.12747
M3 - Journal article
C2 - 27144495
AN - SCOPUS:84984596880
SN - 0954-7894
VL - 46
SP - 1176
EP - 1184
JO - Clinical and Experimental Allergy
JF - Clinical and Experimental Allergy
IS - 9
ER -