TY - JOUR
T1 - Cytotoxic T lymphocytes against the antigen‐processing‐defective RMA‐S tumor cell line
AU - Sijts, Alice J.A.M.
AU - De Bruijn, Marloes L.H.
AU - Nieland, John D.
AU - Kast, W. Martin
AU - Melief, Cornelis J.M.
PY - 1992/6
Y1 - 1992/6
N2 - RMA‐S is an antigen processing‐defective cell line, obtained from a Rauscher virus‐induced tumor. The cells express only a low level of cell surface major histocompatibility complex (MHC) class I molecules, which are supposed to be devoid of internally derived antigenic peptides. We investigated Rauscher virus expression and Rauscher peptide presentation to virus‐specific cytotoxic T lymphocytes (CTL) by this cell line. Viral proteins are expressed properly, both intracellularly and atthe cell surface of RMA‐S. Rauscher peptides are presented tovirus‐specific CTL in the groove of both the class I H2Kb and Db molecules, but at a low level. Culture of RMA‐S cells at room temperature increases theirsusceptibility to CTL. The RMA‐S defect thus affects, but not totally abrogates, Rauscher peptide presentation by MHC class I molecules via the endogenous pathway. Thisindicates that the RMA‐S antigen processing deficit is not absolute.
AB - RMA‐S is an antigen processing‐defective cell line, obtained from a Rauscher virus‐induced tumor. The cells express only a low level of cell surface major histocompatibility complex (MHC) class I molecules, which are supposed to be devoid of internally derived antigenic peptides. We investigated Rauscher virus expression and Rauscher peptide presentation to virus‐specific cytotoxic T lymphocytes (CTL) by this cell line. Viral proteins are expressed properly, both intracellularly and atthe cell surface of RMA‐S. Rauscher peptides are presented tovirus‐specific CTL in the groove of both the class I H2Kb and Db molecules, but at a low level. Culture of RMA‐S cells at room temperature increases theirsusceptibility to CTL. The RMA‐S defect thus affects, but not totally abrogates, Rauscher peptide presentation by MHC class I molecules via the endogenous pathway. Thisindicates that the RMA‐S antigen processing deficit is not absolute.
UR - http://www.scopus.com/inward/record.url?scp=0026539402&partnerID=8YFLogxK
U2 - 10.1002/eji.1830220644
DO - 10.1002/eji.1830220644
M3 - Journal article
C2 - 1601045
AN - SCOPUS:0026539402
SN - 0014-2980
VL - 22
SP - 1639
EP - 1642
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 6
ER -