TY - JOUR
T1 - Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies
T2 - from bioreactors to proteogenomics
AU - Poulsen, Jan Struckmann
AU - Macêdo, Williane Vieira
AU - Bonde, Torben
AU - Nielsen, Jeppe Lund
N1 - © 2023. The Author(s).
PY - 2023/11/28
Y1 - 2023/11/28
N2 - The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being used towards resources recovery, leaving a great potential unexploited. In this study, the combination of state-of-art genomic techniques and engineered systems were used to further advance the knowledge on biogas production from lignocellulosic-rich residues and the microbiome involved in the anaerobic digestion hereof. A long-term adapted anaerobic microbiome capable of degrading wheat straw as the sole substrate was investigated using protein stable isotope probing (protein-SIP). The results indicated that a diverse microbial community, primarily composed of Firmicutes and Methanogens, played crucial roles in cellulose degradation and methane production. Notably, Defluviitoga tunisiensis, Syntrophothermus lipocalidus, and Pelobacter carbinolicus were identified as direct metabolizers of cellulose, while Dehalobacterium assimilated labelled carbon through cross-feeding. This study provides direct evidence of primary cellulose degraders and sheds light on their genomic composition. By harnessing the potential of lignocellulosic biomass and understanding the microbial communities involved, we can promote sustainable biogas production, contributing to energy security and environmental preservation.
AB - The biogas produced through anaerobic digestion (AD) of renewable feedstocks is one of the promising alternatives to replace fossil-derived energy. Even though lignocellulosic biomass is the most abundant biomass on earth, only a small fraction is being used towards resources recovery, leaving a great potential unexploited. In this study, the combination of state-of-art genomic techniques and engineered systems were used to further advance the knowledge on biogas production from lignocellulosic-rich residues and the microbiome involved in the anaerobic digestion hereof. A long-term adapted anaerobic microbiome capable of degrading wheat straw as the sole substrate was investigated using protein stable isotope probing (protein-SIP). The results indicated that a diverse microbial community, primarily composed of Firmicutes and Methanogens, played crucial roles in cellulose degradation and methane production. Notably, Defluviitoga tunisiensis, Syntrophothermus lipocalidus, and Pelobacter carbinolicus were identified as direct metabolizers of cellulose, while Dehalobacterium assimilated labelled carbon through cross-feeding. This study provides direct evidence of primary cellulose degraders and sheds light on their genomic composition. By harnessing the potential of lignocellulosic biomass and understanding the microbial communities involved, we can promote sustainable biogas production, contributing to energy security and environmental preservation.
KW - Anaerobic digestion
KW - Lignocellulose
KW - Metaproteomics
KW - Protein stable isotope probing
UR - http://www.scopus.com/inward/record.url?scp=85178071752&partnerID=8YFLogxK
U2 - 10.1186/s13068-023-02432-x
DO - 10.1186/s13068-023-02432-x
M3 - Journal article
C2 - 38017526
AN - SCOPUS:85178071752
SN - 1754-6834
VL - 16
JO - Biotechnology for Biofuels and Bioproducts
JF - Biotechnology for Biofuels and Bioproducts
IS - 1
M1 - 183
ER -