TY - JOUR
T1 - Evaluation of a commercial competitive enzyme-linked immunosorbent assay for detection of avian influenza virus subtype H5 antibodies in zoo birds
AU - Jensen, Trine Hammer
AU - Andersen, Jannie Holmegaard
AU - Hjulsager, Charlotte Kristiane
AU - Chriél, Mariann
AU - Bertelsen, Mads Frost
PY - 2017/9/1
Y1 - 2017/9/1
N2 - The hemagglutination inhibition (HI) test is the current gold standard for detecting antibodies to avian influenza virus (AIV). Enzyme-linked immunosorbent assays (ELISAs) have been explored for use in poultry and certain wild bird species because of high efficiency and lower cost. This study compared a commercial ELISA for detection of AIV subtype H5 antibodies with HI test of 572 serum samples from zoo birds. There was no significant difference between the results of the two tests when statistically compared by a McNemar χ2 test (P = 0.86) and assessment of κ (κ = 0.87). With a specificity of 94.2% (95% confidence interval CI, 0.92-0.97), a sensitivity of 93.9% (95% CI, 0.91-0.97), and an excellent correlation between the two tests, this ELISA can be recommended as an alternative to the HI test for preliminary screening of zoo bird sera for antibodies to AIV subtype H5.
AB - The hemagglutination inhibition (HI) test is the current gold standard for detecting antibodies to avian influenza virus (AIV). Enzyme-linked immunosorbent assays (ELISAs) have been explored for use in poultry and certain wild bird species because of high efficiency and lower cost. This study compared a commercial ELISA for detection of AIV subtype H5 antibodies with HI test of 572 serum samples from zoo birds. There was no significant difference between the results of the two tests when statistically compared by a McNemar χ2 test (P = 0.86) and assessment of κ (κ = 0.87). With a specificity of 94.2% (95% confidence interval CI, 0.92-0.97), a sensitivity of 93.9% (95% CI, 0.91-0.97), and an excellent correlation between the two tests, this ELISA can be recommended as an alternative to the HI test for preliminary screening of zoo bird sera for antibodies to AIV subtype H5.
KW - Avian influenza
KW - ELISA
KW - H5
KW - hemagglutination inhibition test
KW - zoo birds
UR - http://www.scopus.com/inward/record.url?scp=85029722141&partnerID=8YFLogxK
U2 - 10.1638/2016-0220.1
DO - 10.1638/2016-0220.1
M3 - Journal article
AN - SCOPUS:85029722141
SN - 1042-7260
VL - 48
SP - 882
EP - 885
JO - Journal of Zoo and Wildlife Medicine
JF - Journal of Zoo and Wildlife Medicine
IS - 3
ER -