A fast, economical and efficient method for DNA purification by use of a homemade bead column

N. J.V. Hansen; P. Kristensen; J. Lykke; Kim Kusk Mortensen; B. F.C. Clark

A fast, economical and efficient method for DNA purification by use of a homemade bead column

N. J.V. Hansen^*, P. Kristensen, J. Lykke, Kim Kusk Mortensen, B. F.C. Clark

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

24 Citationer (Scopus)

Abstract

A fast, efficient and economical method for purification of DNA from various sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the method devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable for multiple sample handling in the preparation of DNA for sequencing and other purposes. Protocols are devised for plasmid minipreparation, gel elution and purification of DNA from solutions e.g. enzyme reactions.

Originalsprog	Engelsk
Tidsskrift	Biochemistry and Molecular Biology International
Vol/bind	35
Udgave nummer	3
Sider (fra-til)	461-465
Antal sider	5
ISSN	1039-9712
Status	Udgivet - 1 dec. 1995
Udgivet eksternt	Ja

AUB Link

Søg efter materialet i Aalborg Universitetsbiblioteks søgemaskine

Andre filer og links

http://www.scopus.com/inward/record.url?scp=0029556619&partnerID=8YFLogxK

Citationsformater

@article{9ad98b4c0704447cad386b09fc930991,

title = "A fast, economical and efficient method for DNA purification by use of a homemade bead column",

abstract = "A fast, efficient and economical method for purification of DNA from various sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the method devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable for multiple sample handling in the preparation of DNA for sequencing and other purposes. Protocols are devised for plasmid minipreparation, gel elution and purification of DNA from solutions e.g. enzyme reactions.",

author = "Hansen, {N. J.V.} and P. Kristensen and J. Lykke and Mortensen, {Kim Kusk} and Clark, {B. F.C.}",

year = "1995",

month = dec,

day = "1",

language = "English",

volume = "35",

pages = "461--465",

journal = "Biochemistry and Molecular Biology International",

issn = "1039-9712",

publisher = "Wiley",

number = "3",

}

TY - JOUR

T1 - A fast, economical and efficient method for DNA purification by use of a homemade bead column

AU - Hansen, N. J.V.

AU - Kristensen, P.

AU - Lykke, J.

AU - Mortensen, Kim Kusk

AU - Clark, B. F.C.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - A fast, efficient and economical method for purification of DNA from various sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the method devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable for multiple sample handling in the preparation of DNA for sequencing and other purposes. Protocols are devised for plasmid minipreparation, gel elution and purification of DNA from solutions e.g. enzyme reactions.

AB - A fast, efficient and economical method for purification of DNA from various sources by the use of silica particles packed in homemade bead columns is described. The method is a further development of the method devised by Carter and Milton (1), improved by the introduction of a column operated with a vacuum system. The method is highly suitable for multiple sample handling in the preparation of DNA for sequencing and other purposes. Protocols are devised for plasmid minipreparation, gel elution and purification of DNA from solutions e.g. enzyme reactions.

UR - http://www.scopus.com/inward/record.url?scp=0029556619&partnerID=8YFLogxK

M3 - Journal article

C2 - 7773182

AN - SCOPUS:0029556619

SN - 1039-9712

VL - 35

SP - 461

EP - 465

JO - Biochemistry and Molecular Biology International

JF - Biochemistry and Molecular Biology International

IS - 3

ER -

A fast, economical and efficient method for DNA purification by use of a homemade bead column

Abstract

AUB Link

Andre filer og links

Fingeraftryk

Citationsformater