Abstract
The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transport of L-alanine. In addition to Na+, the transport of L-alanine was influenced by H+. However, H+ does not substitute for Na+, but instead potentiates the Na+ effect. Modification of histidyl residues of the intact luminal-membrane vesicles by diethylpyrocarbonate (DEP), completely abolished the transient renal accumulation of L-alanine. Substrate and Na+-protection experiments suggest that histidyl residues may be at or close to the active site of the L-alanine transporter in membrane vesicles from pars recta.
Originalsprog | Engelsk |
---|---|
Tidsskrift | FEBS Letters |
Vol/bind | 227 |
Udgave nummer | 1 |
Sider (fra-til) | 35-38 |
Antal sider | 4 |
ISSN | 0014-5793 |
DOI | |
Status | Udgivet - 18 jan. 1988 |
Udgivet eksternt | Ja |