TY - JOUR
T1 - The human keratinocyte two-dimensional gel protein database (update 1995)
T2 - mapping components of signal transduction pathways
AU - Celis, Julio E.
AU - Rasmussen, Hanne Holm
AU - Gromov, Paul
AU - Olsen, Eydfinnur
AU - Madsen, Peder
AU - Leffers, Henrik
AU - Honoré, Bent
AU - Dejgaard, Kurt
AU - Vorum, Henrik
AU - Kristensen, Dan Back
AU - Østergaard, Morten
AU - Haunsø, Anders
AU - Jensen, Niels Aagaard
AU - Celis, Ariana
AU - Basse, Bodil
AU - Lauridsen, Jette B.
AU - Ratz, Gitte P.
AU - Andersen, Annette H.
AU - Walbum, Else
AU - Kjærgaard, Inge
AU - Andersen, Inger
AU - Puype, Magda
AU - Van Damme, Josef
AU - Vandekerckhove, Joël
PY - 1995/12
Y1 - 1995/12
N2 - The master two-dimensional (2-D) gel database of human keratinocytes currently lists 3154 cellular proteins (2224 isoelectric focusing, IEF; and 930 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to post-translational modifications. 1082 polypeptides have been identified (protein name, organelle components, etc.) using a procedure or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-D gel immunoblotting using specific antibodies, (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry, (v)vaccinia virus expression of full length cDNAs, and (vi) in vitro transcription/translation of full-length cDNAs. This year, special emphasis has been given to the identification of signal transduction components by using 2-D gel immunoblotting of crude keratinocyte lysates in combination with enhanced chemoluminescence (ECL) detection. Identified proteins are listed both in alphabetical order and with increasing SSP number, together with their M(r), pI, cellular localization and credit to the investigator(s) that aided in the identification. Ultimately, the aim of the comprehensive database is to gather--through a systematic study of ekeratinocytes--qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
AB - The master two-dimensional (2-D) gel database of human keratinocytes currently lists 3154 cellular proteins (2224 isoelectric focusing, IEF; and 930 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to post-translational modifications. 1082 polypeptides have been identified (protein name, organelle components, etc.) using a procedure or a combination of procedures that include (i) comigration with known human proteins, (ii) 2-D gel immunoblotting using specific antibodies, (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry, (v)vaccinia virus expression of full length cDNAs, and (vi) in vitro transcription/translation of full-length cDNAs. This year, special emphasis has been given to the identification of signal transduction components by using 2-D gel immunoblotting of crude keratinocyte lysates in combination with enhanced chemoluminescence (ECL) detection. Identified proteins are listed both in alphabetical order and with increasing SSP number, together with their M(r), pI, cellular localization and credit to the investigator(s) that aided in the identification. Ultimately, the aim of the comprehensive database is to gather--through a systematic study of ekeratinocytes--qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
KW - Cells, Cultured
KW - Chemical Fractionation
KW - Databases, Factual
KW - Electrophoresis, Gel, Two-Dimensional
KW - Epidermal Cells
KW - Humans
KW - Keratinocytes/chemistry
KW - Proteins/analysis
KW - Signal Transduction
U2 - 10.1002/elps.11501601355
DO - 10.1002/elps.11501601355
M3 - Journal article
C2 - 8824782
SN - 0173-0835
VL - 16
SP - 2177
EP - 2240
JO - Electrophoresis
JF - Electrophoresis
IS - 1
ER -