TY - JOUR
T1 - The human keratinocyte two‐dimensional protein database (update 1994)
T2 - Towards an integrated approach to the study of cell proliferation, differentiation and skin diseases
AU - Celis, Julio E.
AU - Rasmussen, Hanne Holm
AU - Olsen, Eydfinnur
AU - Madsen, Peder
AU - Leffers, Henrik
AU - Honoré, Bent
AU - Dejgaard, Kurt
AU - Gromov, Paul
AU - Vorum, Henrik
AU - Vassilev, Alex
AU - Baskin, Yasemin
AU - Liu, Xiangdang
AU - Celis, Ariana
AU - Basse, Bodil
AU - Lauridsen, Jette B.
AU - Ratz, Gitte P.
AU - Andersen, Annette H.
AU - Walbum, Else
AU - Kjærgaard, Inge
AU - Andersen, Inger
AU - Puype, Magda
AU - Van Damme, Josef
AU - Vandekerckhove, Joël
PY - 1994
Y1 - 1994
N2 - The master two‐dimensional (2‐D) gel database of human keratinocytes currently lists 3087 cellular proteins (2168 isoelectric focusing, IEF; and 919 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to posttranslational modifications. 890 polypeptides have been identified (protein name, organelle components, etc.) using one or a combination of procedures that include (i) comigration with known human proteins, (ii) 2‐D gel immunoblotting using specific antibodies (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry and (v) vaccinia virus expression of full length cDNAs. These are listed both in alphabetical order and with increasing SSP number, together with their Mr, pI, cellular localization and credit to the investigator(s) that aided in the identification. Furthermore, we list 239 microsequenced proteins recorded in the database. We also report a database of proteins recovered from the medium of noncultured, unfractionated keratinocytes. This database lists 398 polypeptides (309 IEF; 89 NEPHGE) of which 76 have been identified. The aim of the comprehensive databases is to gather, through a systematic study of keratinocytes, qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and, ultimately, to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
AB - The master two‐dimensional (2‐D) gel database of human keratinocytes currently lists 3087 cellular proteins (2168 isoelectric focusing, IEF; and 919 nonequilibrium pH gradient electrophoresis, NEPHGE), many of which correspond to posttranslational modifications. 890 polypeptides have been identified (protein name, organelle components, etc.) using one or a combination of procedures that include (i) comigration with known human proteins, (ii) 2‐D gel immunoblotting using specific antibodies (iii) microsequencing of Coomassie Brilliant Blue stained proteins, (iv) mass spectrometry and (v) vaccinia virus expression of full length cDNAs. These are listed both in alphabetical order and with increasing SSP number, together with their Mr, pI, cellular localization and credit to the investigator(s) that aided in the identification. Furthermore, we list 239 microsequenced proteins recorded in the database. We also report a database of proteins recovered from the medium of noncultured, unfractionated keratinocytes. This database lists 398 polypeptides (309 IEF; 89 NEPHGE) of which 76 have been identified. The aim of the comprehensive databases is to gather, through a systematic study of keratinocytes, qualitative and quantitative information on proteins and their genes that may allow us to identify abnormal patterns of gene expression and, ultimately, to pinpoint signaling pathways and components affected in various skin diseases, cancer included.
U2 - 10.1002/elps.11501501208
DO - 10.1002/elps.11501501208
M3 - Journal article
C2 - 7895731
AN - SCOPUS:0028147242
SN - 0173-0835
VL - 15
SP - 1349
EP - 1458
JO - Electrophoresis
JF - Electrophoresis
IS - 1
ER -