Comparison of RNA-Seq and microarray analysis with qRT-PCR validation assays. Quantitative measurement of gene expression was determined with qRT-PCR for 15 DEGs in microarray (A) and 13 DEGs in RNA-Seq results (B). Data were obtained from three independent cDNA sets from three independent experiments, normalized to eukaryotic elongation factor 5A3 and expressed as the means of log2 (ΔΔCt) ± SEM (standard error of the mean). The statistical analysis for the data was performed with coefficient correlation analysis between microarray or RNA-Seq data and qRT-PCR data (log2 fold change) analyzed by the Pearson test (P
Date made available | 17 Jun 2019 |
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Publisher | Figshare |
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