Abstract
Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log10-reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.
Original language | English |
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Journal | Wound Repair and Regeneration |
Volume | 29 |
Issue number | 5 |
Pages (from-to) | 820-829 |
Number of pages | 10 |
ISSN | 1067-1927 |
DOIs | |
Publication status | Published - 1 Sept 2021 |
Bibliographical note
Funding Information:The China Scholarship Council has funded the PhD study of Xiaofeng Chen. Ida C. Thaarup is funded by Magle Chemoswed AB, through a non‐restricted PhD research grant. The funding sources were not involved in the study design, in the collection, analysis and interpretation of data, in the writing of the report, or in the decision to submit the article for publication. Kim Tanja Hejselbak Nørgaard is acknowledged for making the illustrations of the model. Susanne Bielidt is acknowledged for her support in the laboratory.
Publisher Copyright:
© 2021 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.
Keywords
- biofilm
- coexistence of Pseudomonas aeruginosa and
- model
- Staphylococcus aureus
- test of antibiofilm effect