TY - JOUR
T1 - Early life DNA vaccination with the H gene of Canine distemper virus induces robust protection against distemper
AU - Jensen, Trine Hammer
AU - Nielsen, Line
AU - Aasted, Bent
AU - Blixenkrone-Møller, Merete
N1 - Funding Information:
The Danish Fur Breeders Research Fund and Faculty of Life Sciences, University of Copenhagen are acknowledged for their financial support. We wish to thank Leon Langli, Jørgen Østergaard and Klaus Hartmann for help with handling and care of the mink and Tove Dannemann Jensen, Alma Orantes and Morten Lund for excellent technical assistance.
PY - 2009/8/20
Y1 - 2009/8/20
N2 - Young mink kits (n = 8) were vaccinated with DNA plasmids encoding the viral haemagglutinin protein (H) of a vaccine strain of Canine distemper virus (CDV). Virus neutralising (VN) antibodies were induced after 2 immunisations and after the third immunisation all kits had high VN antibody titres. The VN antibody titres remained high for more than 4 months and the mink were protected against viraemia, lymphopenia, clinical disease and changes in the percentage of IFN-γ producing peripheral blood leucocytes after challenge inoculation with a recent wild type strain of CDV. Essentially, these results demonstrate that early life DNA vaccination with the H gene of a CDV vaccine strain induced robust protective immunity against a recent wild type CDV.
AB - Young mink kits (n = 8) were vaccinated with DNA plasmids encoding the viral haemagglutinin protein (H) of a vaccine strain of Canine distemper virus (CDV). Virus neutralising (VN) antibodies were induced after 2 immunisations and after the third immunisation all kits had high VN antibody titres. The VN antibody titres remained high for more than 4 months and the mink were protected against viraemia, lymphopenia, clinical disease and changes in the percentage of IFN-γ producing peripheral blood leucocytes after challenge inoculation with a recent wild type strain of CDV. Essentially, these results demonstrate that early life DNA vaccination with the H gene of a CDV vaccine strain induced robust protective immunity against a recent wild type CDV.
KW - Canine distemper virus
KW - DNA vaccination
KW - Early life vaccination
UR - http://www.scopus.com/inward/record.url?scp=67651083737&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2009.06.077
DO - 10.1016/j.vaccine.2009.06.077
M3 - Journal article
C2 - 19596418
AN - SCOPUS:67651083737
SN - 0264-410X
VL - 27
SP - 5178
EP - 5183
JO - Vaccine
JF - Vaccine
IS - 38
ER -