Abstract
Nanoporous gold (NPG) based biosensors have been constructed by covalently immobilizing glucose oxidase (GOx) onto self-assembled monolayers (SAMs). With p-benzoquinone (BQ) as a mediator, diffusion behavior and amperometric biosensor performance are evaluated by electrochemical characterization. The enzyme modified electrodes are demonstrated to show a thickness-sensitive behavior. Compared with planar polycrystalline gold, the unique porous structure of NPG has also been characterized via an electrochemical surface reconstruction process. Single-crystal gold-like electrochemical behavior on NPG and a comprehensive understanding of its impacts on sensor performance have been proposed.
Original language | English |
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Journal | Analyst |
Volume | 139 |
Issue number | 2 |
Pages (from-to) | 488-494 |
Number of pages | 7 |
ISSN | 0003-2654 |
DOIs | |
Publication status | Published - 9 Dec 2013 |