Fermentation in 24-well plates is an efficient screening platform for filamentous fungi

Tore Linde, Bjørn Hansen, Mette Lübeck, Peter Stephensen Lübeck

Research output: Contribution to conference without publisher/journalPosterResearch

Abstract

Conventional citric acid batch-fermentation by filamentous fungi is often linked with large variations in production levels. The most used citric acid batch-fermentation method described in literature is based on Erlenmeyer flasks with cotton-stoppers in a heated shaker. Our preliminary studies using Erlenmeyer flasks in sextuplicates showed a relative standard sample deviation of 54 % citric acid produced with the filamentous fungi Aspergillus carbonarius, thereby confirming these large variations. The objective of this study was to propose a different and more reliable screening method for citric acid production from filamentous fungi, using A. carbonarius as test organism in a 24 well plate system. The two systems where run simultaneously with only the mechanical parameters as difference between them. All experiments were performed in sextuplicates to be able to give a higher statistical impact than the conventional triplicates. After 5 days fermentation, the citric acid produced in the Erlenmeyer system had a relative standard deviation of 48% compared to the 24-well system that gave a relative standard deviation of 6%. The statistical analysis showed that the difference between the replicates in the 24 well plate system was significantly smaller than in the conventional Erlenmeyer flask setup. This allows for a more precise determination of effects caused by for example changing fermentation parameters or making genetic alterations. We therefore conclude that fermentation in 24-well plates is a more reliable screening method for citric acid production by filamentous fungi, and likely for screening in general. Furthermore by screening in a 24 well plate system, it is possible to screen the same amount of samples as in a conventional flask system, using less space and less materials
Original languageEnglish
Publication date22 Mar 2014
Number of pages1
Publication statusPublished - 22 Mar 2014
EventEuropean Conference on Fungal Genetics : Fungal genetics - Silken Al-Andalus Palace Hotel, Sevilla, Spain
Duration: 22 Apr 201427 Apr 2014
Conference number: 12

Conference

ConferenceEuropean Conference on Fungal Genetics
Number12
LocationSilken Al-Andalus Palace Hotel
CountrySpain
CitySevilla
Period22/04/201427/04/2014

Cite this

Linde, T., Hansen, B., Lübeck, M., & Lübeck, P. S. (2014). Fermentation in 24-well plates is an efficient screening platform for filamentous fungi. Poster presented at European Conference on Fungal Genetics , Sevilla, Spain.
Linde, Tore ; Hansen, Bjørn ; Lübeck, Mette ; Lübeck, Peter Stephensen. / Fermentation in 24-well plates is an efficient screening platform for filamentous fungi. Poster presented at European Conference on Fungal Genetics , Sevilla, Spain.1 p.
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abstract = "Conventional citric acid batch-fermentation by filamentous fungi is often linked with large variations in production levels. The most used citric acid batch-fermentation method described in literature is based on Erlenmeyer flasks with cotton-stoppers in a heated shaker. Our preliminary studies using Erlenmeyer flasks in sextuplicates showed a relative standard sample deviation of 54 {\%} citric acid produced with the filamentous fungi Aspergillus carbonarius, thereby confirming these large variations. The objective of this study was to propose a different and more reliable screening method for citric acid production from filamentous fungi, using A. carbonarius as test organism in a 24 well plate system. The two systems where run simultaneously with only the mechanical parameters as difference between them. All experiments were performed in sextuplicates to be able to give a higher statistical impact than the conventional triplicates. After 5 days fermentation, the citric acid produced in the Erlenmeyer system had a relative standard deviation of 48{\%} compared to the 24-well system that gave a relative standard deviation of 6{\%}. The statistical analysis showed that the difference between the replicates in the 24 well plate system was significantly smaller than in the conventional Erlenmeyer flask setup. This allows for a more precise determination of effects caused by for example changing fermentation parameters or making genetic alterations. We therefore conclude that fermentation in 24-well plates is a more reliable screening method for citric acid production by filamentous fungi, and likely for screening in general. Furthermore by screening in a 24 well plate system, it is possible to screen the same amount of samples as in a conventional flask system, using less space and less materials",
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Fermentation in 24-well plates is an efficient screening platform for filamentous fungi. / Linde, Tore; Hansen, Bjørn; Lübeck, Mette; Lübeck, Peter Stephensen.

2014. Poster presented at European Conference on Fungal Genetics , Sevilla, Spain.

Research output: Contribution to conference without publisher/journalPosterResearch

TY - CONF

T1 - Fermentation in 24-well plates is an efficient screening platform for filamentous fungi

AU - Linde, Tore

AU - Hansen, Bjørn

AU - Lübeck, Mette

AU - Lübeck, Peter Stephensen

PY - 2014/3/22

Y1 - 2014/3/22

N2 - Conventional citric acid batch-fermentation by filamentous fungi is often linked with large variations in production levels. The most used citric acid batch-fermentation method described in literature is based on Erlenmeyer flasks with cotton-stoppers in a heated shaker. Our preliminary studies using Erlenmeyer flasks in sextuplicates showed a relative standard sample deviation of 54 % citric acid produced with the filamentous fungi Aspergillus carbonarius, thereby confirming these large variations. The objective of this study was to propose a different and more reliable screening method for citric acid production from filamentous fungi, using A. carbonarius as test organism in a 24 well plate system. The two systems where run simultaneously with only the mechanical parameters as difference between them. All experiments were performed in sextuplicates to be able to give a higher statistical impact than the conventional triplicates. After 5 days fermentation, the citric acid produced in the Erlenmeyer system had a relative standard deviation of 48% compared to the 24-well system that gave a relative standard deviation of 6%. The statistical analysis showed that the difference between the replicates in the 24 well plate system was significantly smaller than in the conventional Erlenmeyer flask setup. This allows for a more precise determination of effects caused by for example changing fermentation parameters or making genetic alterations. We therefore conclude that fermentation in 24-well plates is a more reliable screening method for citric acid production by filamentous fungi, and likely for screening in general. Furthermore by screening in a 24 well plate system, it is possible to screen the same amount of samples as in a conventional flask system, using less space and less materials

AB - Conventional citric acid batch-fermentation by filamentous fungi is often linked with large variations in production levels. The most used citric acid batch-fermentation method described in literature is based on Erlenmeyer flasks with cotton-stoppers in a heated shaker. Our preliminary studies using Erlenmeyer flasks in sextuplicates showed a relative standard sample deviation of 54 % citric acid produced with the filamentous fungi Aspergillus carbonarius, thereby confirming these large variations. The objective of this study was to propose a different and more reliable screening method for citric acid production from filamentous fungi, using A. carbonarius as test organism in a 24 well plate system. The two systems where run simultaneously with only the mechanical parameters as difference between them. All experiments were performed in sextuplicates to be able to give a higher statistical impact than the conventional triplicates. After 5 days fermentation, the citric acid produced in the Erlenmeyer system had a relative standard deviation of 48% compared to the 24-well system that gave a relative standard deviation of 6%. The statistical analysis showed that the difference between the replicates in the 24 well plate system was significantly smaller than in the conventional Erlenmeyer flask setup. This allows for a more precise determination of effects caused by for example changing fermentation parameters or making genetic alterations. We therefore conclude that fermentation in 24-well plates is a more reliable screening method for citric acid production by filamentous fungi, and likely for screening in general. Furthermore by screening in a 24 well plate system, it is possible to screen the same amount of samples as in a conventional flask system, using less space and less materials

KW - Fermentering

KW - Optimering

KW - filamentiøse svampe

M3 - Poster

ER -

Linde T, Hansen B, Lübeck M, Lübeck PS. Fermentation in 24-well plates is an efficient screening platform for filamentous fungi. 2014. Poster presented at European Conference on Fungal Genetics , Sevilla, Spain.