In vitro infection of human placental trophoblast by wild-type vaccinia virus and recombinant virus expressing HIV envelope glycoprotein

N. Nørskov-Lauritsen; V. Zachar; P. M. Petersen; H. Hager; G. Aboagye-Mathiesen; P. Ebbesen

doi:10.1016/S0923-2516(06)80120-2

In vitro infection of human placental trophoblast by wild-type vaccinia virus and recombinant virus expressing HIV envelope glycoprotein

N. Nørskov-Lauritsen, V. Zachar, P. M. Petersen, H. Hager, G. Aboagye-Mathiesen, P. Ebbesen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

9 Citations (Scopus)

Abstract

Short-time (≤7 days) cultures of trophoblast mononuclear cells isolated from term placentae were challenged with vaccinia virus. Cytopathic effects were induced in crude placental cell preparations as well as in cultures established after negative immunosorting of major histocompatibility complex class I epitope-expressing cells, i.e. cultures exclusively derived from villous cytotrophoblast according to our present state of knowledge. The trophoblast in vitro supported a full replicative cycle of both wild-type viruses and a recombinant clone serving as a vector for the human immunodeficiency virus type 1 envelope gene. Results may shed light on mechanisms involved in the rarely observed foetal damage caused by smallpox vaccination during pregnancy.

Original language	English
Journal	Research in Virology
Volume	143
Issue number	C
Pages (from-to)	321-328
Number of pages	8
ISSN	0923-2516
DOIs	https://doi.org/10.1016/S0923-2516(06)80120-2
Publication status	Published - 1992

Keywords

AIDS
Foetus
Recombinant vaccinia virus
Transplacental virus transmission
Vaccination
Vaccinia

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title = "In vitro infection of human placental trophoblast by wild-type vaccinia virus and recombinant virus expressing HIV envelope glycoprotein",

abstract = "Short-time (≤7 days) cultures of trophoblast mononuclear cells isolated from term placentae were challenged with vaccinia virus. Cytopathic effects were induced in crude placental cell preparations as well as in cultures established after negative immunosorting of major histocompatibility complex class I epitope-expressing cells, i.e. cultures exclusively derived from villous cytotrophoblast according to our present state of knowledge. The trophoblast in vitro supported a full replicative cycle of both wild-type viruses and a recombinant clone serving as a vector for the human immunodeficiency virus type 1 envelope gene. Results may shed light on mechanisms involved in the rarely observed foetal damage caused by smallpox vaccination during pregnancy.",

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T1 - In vitro infection of human placental trophoblast by wild-type vaccinia virus and recombinant virus expressing HIV envelope glycoprotein

AU - Nørskov-Lauritsen, N.

AU - Zachar, V.

AU - Petersen, P. M.

AU - Hager, H.

AU - Aboagye-Mathiesen, G.

AU - Ebbesen, P.

PY - 1992

Y1 - 1992

N2 - Short-time (≤7 days) cultures of trophoblast mononuclear cells isolated from term placentae were challenged with vaccinia virus. Cytopathic effects were induced in crude placental cell preparations as well as in cultures established after negative immunosorting of major histocompatibility complex class I epitope-expressing cells, i.e. cultures exclusively derived from villous cytotrophoblast according to our present state of knowledge. The trophoblast in vitro supported a full replicative cycle of both wild-type viruses and a recombinant clone serving as a vector for the human immunodeficiency virus type 1 envelope gene. Results may shed light on mechanisms involved in the rarely observed foetal damage caused by smallpox vaccination during pregnancy.

AB - Short-time (≤7 days) cultures of trophoblast mononuclear cells isolated from term placentae were challenged with vaccinia virus. Cytopathic effects were induced in crude placental cell preparations as well as in cultures established after negative immunosorting of major histocompatibility complex class I epitope-expressing cells, i.e. cultures exclusively derived from villous cytotrophoblast according to our present state of knowledge. The trophoblast in vitro supported a full replicative cycle of both wild-type viruses and a recombinant clone serving as a vector for the human immunodeficiency virus type 1 envelope gene. Results may shed light on mechanisms involved in the rarely observed foetal damage caused by smallpox vaccination during pregnancy.

KW - AIDS

KW - Foetus

KW - Recombinant vaccinia virus

KW - Transplacental virus transmission

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AN - SCOPUS:0026621202

SN - 0923-2516

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JO - Research in Virology

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In vitro infection of human placental trophoblast by wild-type vaccinia virus and recombinant virus expressing HIV envelope glycoprotein

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