Large-Scale Protein Analysis of Experimental Retinal Artery Occlusion

Nanna Vestergaard*, Lasse Jørgensen Cehofski, Alexander Nørgård Alsing, Anders Kruse, Jonas Ellegaard Nielsen, Anders Schlosser, Grith Lykke Sorensen, Bent Honoré, Henrik Vorum

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Retinal artery occlusion (RAO) is a devastating condition with no effective treatment. The management of RAO could potentially be improved through an in-depth understanding of the molecular alterations in the condition. This study combined advanced proteomic techniques and an experimental model to uncover the retinal large-scale protein profile of RAO. In 13 pigs, RAO was induced with an argon laser and confirmed by fluorescein angiography. Left eyes serving as controls received a sham laser without inducing occlusion. Retinal samples were collected after one, three, or six days and analyzed with liquid chromatography—tandem mass spectrometry. In RAO, 36 proteins were differentially regulated on day one, 86 on day three, and 557 on day six. Upregulated proteins included clusterin, vitronectin, and vimentin, with several proteins increasing over time with a maximum on day six, including clusterin, vimentin, osteopontin, annexin-A, signal transducer, and the activator of transcription 3. On day six, RAO resulted in the upregulation of proteins involved in cellular response to stress, hemostasis, innate immune response, and cytokine signaling. Downregulated proteins were involved in transmission across chemical synapses and visual phototransduction. This study identified the upregulation of multiple inflammatory proteins in RAO and the downregulation of proteins involved in visual pathways.
Original languageEnglish
Article number7919
JournalInternational Journal of Molecular Sciences
Volume24
Issue number9
ISSN1422-0067
DOIs
Publication statusPublished - May 2023

Keywords

  • Animals
  • Clusterin
  • Proteomics/methods
  • Retina
  • Retinal Artery Occlusion
  • Swine
  • Vimentin/genetics
  • animal models
  • proteomics
  • retinal artery occlusion
  • mass spectrometry
  • retinal ischemia diseases

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