Vaccinia virus infection of cultured human first trimester trophoblast

Niels Nørskov-Lauritsen; George Aboagye-Mathiesen; Anne M. Dalsgaard; Anne Gildberg; Peter M. Petersen; Ferenc D. Tóth; Vladimir Zachar; Peter Ebbesen

doi:10.1016/S0143-4004(05)80341-8

Vaccinia virus infection of cultured human first trimester trophoblast

Niels Nørskov-Lauritsen, George Aboagye-Mathiesen, Anne M. Dalsgaard, Anne Gildberg, Peter M. Petersen, Ferenc D. Tóth, Vladimir Zachar, Peter Ebbesen^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Congenital vaccinia is a well known cause of fetal wastage. To investigate a possible mechanism of virus transmission from mother to fetus, human trophoblast isolated from 7–12 weeks placentae was challenged with vaccinia virus. First trimester trophoblast was susceptible to vaccinia virus infection in vitro, as determined by morphologic alterations, cessation of hCG synthesis, and production of progeny virus. A recombinant virus carrying HIV DNA sequences induced trophoblast expression of gp 160 and replicated to the same extent as the control wild-type strain. The results may have a bearing on the general pathogenesis of fetal infections, and serve as a reminder of the small but not negligible risk of vaccination with live virus in pregnancy.

Original language	English
Journal	Placenta
Volume	15
Pages (from-to)	151-160
Number of pages	10
ISSN	0143-4004
DOIs	https://doi.org/10.1016/S0143-4004(05)80341-8
Publication status	Published - 1994

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title = "Vaccinia virus infection of cultured human first trimester trophoblast",

abstract = "Congenital vaccinia is a well known cause of fetal wastage. To investigate a possible mechanism of virus transmission from mother to fetus, human trophoblast isolated from 7–12 weeks placentae was challenged with vaccinia virus. First trimester trophoblast was susceptible to vaccinia virus infection in vitro, as determined by morphologic alterations, cessation of hCG synthesis, and production of progeny virus. A recombinant virus carrying HIV DNA sequences induced trophoblast expression of gp 160 and replicated to the same extent as the control wild-type strain. The results may have a bearing on the general pathogenesis of fetal infections, and serve as a reminder of the small but not negligible risk of vaccination with live virus in pregnancy.",

author = "Niels N{\o}rskov-Lauritsen and George Aboagye-Mathiesen and Dalsgaard, {Anne M.} and Anne Gildberg and Petersen, {Peter M.} and T{\'o}th, {Ferenc D.} and Vladimir Zachar and Peter Ebbesen",

year = "1994",

doi = "10.1016/S0143-4004(05)80341-8",

language = "English",

volume = "15",

pages = "151--160",

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TY - JOUR

T1 - Vaccinia virus infection of cultured human first trimester trophoblast

AU - Nørskov-Lauritsen, Niels

AU - Aboagye-Mathiesen, George

AU - Dalsgaard, Anne M.

AU - Gildberg, Anne

AU - Petersen, Peter M.

AU - Tóth, Ferenc D.

AU - Zachar, Vladimir

AU - Ebbesen, Peter

PY - 1994

Y1 - 1994

N2 - Congenital vaccinia is a well known cause of fetal wastage. To investigate a possible mechanism of virus transmission from mother to fetus, human trophoblast isolated from 7–12 weeks placentae was challenged with vaccinia virus. First trimester trophoblast was susceptible to vaccinia virus infection in vitro, as determined by morphologic alterations, cessation of hCG synthesis, and production of progeny virus. A recombinant virus carrying HIV DNA sequences induced trophoblast expression of gp 160 and replicated to the same extent as the control wild-type strain. The results may have a bearing on the general pathogenesis of fetal infections, and serve as a reminder of the small but not negligible risk of vaccination with live virus in pregnancy.

AB - Congenital vaccinia is a well known cause of fetal wastage. To investigate a possible mechanism of virus transmission from mother to fetus, human trophoblast isolated from 7–12 weeks placentae was challenged with vaccinia virus. First trimester trophoblast was susceptible to vaccinia virus infection in vitro, as determined by morphologic alterations, cessation of hCG synthesis, and production of progeny virus. A recombinant virus carrying HIV DNA sequences induced trophoblast expression of gp 160 and replicated to the same extent as the control wild-type strain. The results may have a bearing on the general pathogenesis of fetal infections, and serve as a reminder of the small but not negligible risk of vaccination with live virus in pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=85025418487&partnerID=8YFLogxK

U2 - 10.1016/S0143-4004(05)80341-8

DO - 10.1016/S0143-4004(05)80341-8

M3 - Journal article

AN - SCOPUS:85025418487

SN - 0143-4004

VL - 15

SP - 151

EP - 160

JO - Placenta

JF - Placenta

ER -

Vaccinia virus infection of cultured human first trimester trophoblast

Abstract

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