Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

Kurt Fuursted; Lone Schøler; Karen Dam; Martin S Bojer; Anette M Hammerum; Frederik Dagnæs-Hansen; Anders Olsen; Yousef Jasemian; Carsten Struve

doi:10.1016/j.micinf.2011.08.015

Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

Kurt Fuursted, Lone Schøler, Karen Dam, Martin S Bojer, Anette M Hammerum, Frederik Dagnæs-Hansen, Anders Olsen, Yousef Jasemian, Carsten Struve

Research output: Contribution to journal › Journal article › Research › peer-review

32 Citations (Scopus)

Abstract

The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.

Original language	English
Journal	Microbes and Infection
Volume	14
Issue number	2
Pages (from-to)	155-8
Number of pages	4
ISSN	1286-4579
DOIs	https://doi.org/10.1016/j.micinf.2011.08.015
Publication status	Published - Feb 2012
Externally published	Yes

Keywords

Animals
Anti-Bacterial Agents/pharmacology
Biofilms/growth & development
Body Temperature
Caenorhabditis elegans/microbiology
Drug Resistance, Multiple, Bacterial
Female
Humans
Kaplan-Meier Estimate
Klebsiella Infections/microbiology
Klebsiella pneumoniae/enzymology
Mice
Mice, Inbred C57BL
Microbial Sensitivity Tests
Models, Animal
Sepsis/microbiology
Virulence/genetics
beta-Lactamases/genetics

Access to Document

10.1016/j.micinf.2011.08.015

AUB Link

Search for the material in Aalborg University Library's search engine

Cite this

@article{66d3d5d342a2418f83c715dc001e4c6e,

title = "Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)",

abstract = "The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.",

keywords = "Animals, Anti-Bacterial Agents/pharmacology, Biofilms/growth & development, Body Temperature, Caenorhabditis elegans/microbiology, Drug Resistance, Multiple, Bacterial, Female, Humans, Kaplan-Meier Estimate, Klebsiella Infections/microbiology, Klebsiella pneumoniae/enzymology, Mice, Mice, Inbred C57BL, Microbial Sensitivity Tests, Models, Animal, Sepsis/microbiology, Virulence/genetics, beta-Lactamases/genetics",

author = "Kurt Fuursted and Lone Sch{\o}ler and Karen Dam and Bojer, {Martin S} and Hammerum, {Anette M} and Frederik Dagn{\ae}s-Hansen and Anders Olsen and Yousef Jasemian and Carsten Struve",

year = "2012",

month = feb,

doi = "10.1016/j.micinf.2011.08.015",

language = "English",

volume = "14",

pages = "155--8",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

AU - Fuursted, Kurt

AU - Schøler, Lone

AU - Dam, Karen

AU - Bojer, Martin S

AU - Hammerum, Anette M

AU - Dagnæs-Hansen, Frederik

AU - Olsen, Anders

AU - Jasemian, Yousef

AU - Struve, Carsten

PY - 2012/2

Y1 - 2012/2

N2 - The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.

AB - The aim of the study was to compare and evaluate virulence in five strains of Klebsiella pneumoniae, including an isolate carrying New Delhi metallo-beta-lactamase-1 (NDM-1). In vivo virulence was assessed using a murine sepsis model and using the nematode Caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. The NDM-1 carrying K. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro virulence factors or killing in C. elegans. It is concluded that K. pneumoniae carrying NDM-1 have an intrinsic virulence potential, which in coexistence with its multiresistance could promote and partly explain its epidemiological success.

KW - Animals

KW - Anti-Bacterial Agents/pharmacology

KW - Biofilms/growth & development

KW - Body Temperature

KW - Caenorhabditis elegans/microbiology

KW - Drug Resistance, Multiple, Bacterial

KW - Female

KW - Humans

KW - Kaplan-Meier Estimate

KW - Klebsiella Infections/microbiology

KW - Klebsiella pneumoniae/enzymology

KW - Mice

KW - Mice, Inbred C57BL

KW - Microbial Sensitivity Tests

KW - Models, Animal

KW - Sepsis/microbiology

KW - Virulence/genetics

KW - beta-Lactamases/genetics

U2 - 10.1016/j.micinf.2011.08.015

DO - 10.1016/j.micinf.2011.08.015

M3 - Journal article

C2 - 21925284

SN - 1286-4579

VL - 14

SP - 155

EP - 158

JO - Microbes and Infection

JF - Microbes and Infection

IS - 2

ER -

Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1)

Abstract

Keywords

Access to Document

AUB Link

Fingerprint

Cite this