Discriminatory rapid tests cause HIV-type misclassification-evaluation of three rapid tests using clinical samples from Guinea-Bissau

Bo Langhoff Hønge, Sanne Jespersen, Candida Medina, David da Silva Té, Zacarias José da Silva, Mette Christiansen, Bertram Kjerulff, Alex Lund Laursen, Christian Wejse, Henrik Krarup, Christian Erikstrup, Bissau HIV Cohort study group

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1 Citationer (Scopus)

Abstract

BACKGROUND: Discrimination among HIV types is important because HIV-2 is naturally resistant to some of the first-line drugs used in the treatment of HIV-1. We evaluated three assays for HIV-type discriminatory capacity: SD Bioline HIV 1/2 3.0 (Bioline), First Response HIV 1-2-0 Card Test (First Response) and Genie III HIV-1/HIV-2 (Genie III).

METHODS: Based on results from the Bioline assay, samples from 239 HIV-infected patients from the Bissau HIV cohort in Guinea-Bissau were retrospectively selected for evaluation. Genie III and First Response were scored by three independent readers and compared with a reference test (INNO-LIA HIV I/II Score) confirmed by HIV RNA as well as DNA detection.

RESULTS: The best performing test was Genie III, with an average agreement with the reference test of 93.4%, followed by First Response (86.1%) and Bioline (72.4%). First Response and Bioline were scored with a false high number of HIV-1/2 dual infections. For both First Response and Genie III, there were discrepancies among independent readers, and some tests were scored as HIV non-reactive.

CONCLUSIONS: Using these rapid tests with a suboptimal performance will presumably result in a high rate of false HIV-1/2 dual diagnoses, depriving patients of alternative treatment options in cases of treatment failure.

OriginalsprogEngelsk
TidsskriftTransactions of the Royal Society of Tropical Medicine and Hygiene
Vol/bind113
Udgave nummer9
Sider (fra-til)555-559
Antal sider5
ISSN0035-9203
DOI
StatusUdgivet - sep. 2019

Bibliografisk note

© The Author(s) 2019. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

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