TY - JOUR
T1 - Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake
AU - Bekker, Nicolai Sundgaard
AU - Toor, Saqib Sohail
AU - Sharma, Kamaldeep
AU - Pedersen, Thomas Helmer
AU - Pedersen, Lars Haastrup
N1 - Publisher Copyright:
© 2023
© 2023 Published by Elsevier Ltd.
PY - 2023/8
Y1 - 2023/8
N2 - In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment. The optimal pretreatment conditions were identified as 190 °C and 10 min resulting in monosaccharide yields of 90% and 73% of the theoretical maximum by slurry and solid conversion, respectively. At optimal conditions, the C6 monosaccharide yield (83–90%) was fairly equal compared to the C5 monosaccharide yield (56–89%), which increased by slurry conversion due to near-complete monomerization of soluble xylo-oligosaccharides. In this study, we showed that clover-grass press cake possesses considerable potential as feedstock for production of fermentable sugars in a biorefinery context.
AB - In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment. The optimal pretreatment conditions were identified as 190 °C and 10 min resulting in monosaccharide yields of 90% and 73% of the theoretical maximum by slurry and solid conversion, respectively. At optimal conditions, the C6 monosaccharide yield (83–90%) was fairly equal compared to the C5 monosaccharide yield (56–89%), which increased by slurry conversion due to near-complete monomerization of soluble xylo-oligosaccharides. In this study, we showed that clover-grass press cake possesses considerable potential as feedstock for production of fermentable sugars in a biorefinery context.
KW - Agro-industrial residues
KW - Enzymatic hydrolysis
KW - Grass pulp
KW - Green biorefinery
KW - Liquid hot water
KW - Monosaccharides
UR - http://www.scopus.com/inward/record.url?scp=85165257927&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2023.e18448
DO - 10.1016/j.heliyon.2023.e18448
M3 - Journal article
C2 - 37534002
AN - SCOPUS:85165257927
SN - 2405-8440
VL - 9
JO - Heliyon
JF - Heliyon
IS - 8
M1 - e18448
ER -